Expression of the neurokinin 1 receptor in the rabbit retina

被引:20
作者
Casini, G [1 ]
Sabatini, A
Catalani, E
Willems, D
Bosco, L
Brecha, NC
机构
[1] Univ Tuscia, Dipartimento Sci Ambientali, I-01100 Viterbo, Italy
[2] Univ Calif Los Angeles, Sch Med, Dept Neurobiol, Jules Stein Eye Inst, Los Angeles, CA 90095 USA
[3] Univ Calif Los Angeles, Sch Med, Dept Med, Jules Stein Eye Inst, Los Angeles, CA 90095 USA
[4] VAGLAHS, Los Angeles, CA 90073 USA
关键词
substance P; dopamine; bipolar cells; amacrine cells; neuropeptide receptors; photoreceptors;
D O I
10.1016/S0306-4522(02)00408-6
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Substance P is the preferred ligand for the neurokinin 1 (NK1) receptor. In vertebrate retinas, substance P is expressed by amacrine, interplexiform and ganglion cells. Substance P influences the activity of amacrine and ganglion cells and it is reported to evoke dopamine release. We investigated NK1 receptor expression in the rabbit retina using affinity-purified NK1 receptor antibodies. NK1 receptors were expressed by two distinct populations of retinal neurons. One is a population of ON-type bipolar cells characterized by axonal arborizations that ramified in the inner plexiform layer near the ganglion cell layer. Double-label studies showed that NK1 receptor-expressing bipolar cells were distinct from rod bipolar cells and from other immunocytochemically identified types of cone bipolar cells. Their density was about 2250 cells/mm(2) in the visual streak and 1115 cells/mm(2) in ventral mid-periphery. They were distributed in a non-random pattern. In the outer plexiform layer, the dendrites of these bipolar cells converged into heavily immunostained clusters having a punctate appearance. The density of these clusters in mid-peripheral ventral regions (about 13000 clusters/mm(2)) was similar to the reported cone density [Famiglietti and Sharpe (1995) Vis. Neurosci. 12, 1151-1175], suggesting these dendrites contact all cone photoreceptors. The second NK1 receptor expressing cell population corresponds to the tyrosine hydroxylase-containing amacrine cell population. NK1 receptor immunostaining was localized to the cell body and processes, but not to the processes that form the 'rings' that are known to encircle somata of All amacrine cells. These findings show that NK1 receptor immunoreactivity is localized to a population of ON-type cone bipolar cells and to dopaminergic amacrine cells, suggesting that substance P acting on NK1 receptors influences multiple retinal circuits in the rabbit retina. (C) 2002 IBRO. Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:1309 / 1321
页数:13
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