Detection of nucleic acids using enzyme-catalyzed template-guided deposition of polyaniline

被引：60

作者：

Gao, Zhiqiang ^{[1
]}

Rafea, Siti ^{[1
]}

Lim, Lay Har ^{[1
]}

机构：

[1] Inst Microelect, Singapore 117685, Singapore

来源：

ADVANCED MATERIALS | 2007年 / 19卷 / 04期

关键词：

D O I：

10.1002/adma.200601090

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An electrochemical procedure for the detection of nucleic acids is realized byutilizing polyaniline as a signal generator for the transduction of nucleic acid hybridization events (see figure). The unique combination of enzymatic amplification and template-guided deposition can be used in conjunction with other detection techniques, and the sensitivity of the biosensor increases with increasing time.

引用

页码：602 / +

页数：6

共 29 条

[1] [Anonymous], 1992, RECOMBINANT DNA
[2] Quantitative analysis of mRNA amplification by in vitro transcription
Baugh, L. R.
Hill, A. A.
Brown, E. L.
Hunter, Craig P.
[J]. NUCLEIC ACIDS RESEARCH, 2001, 29 (05)
[3] Prime time for real-time PCR
Bonetta, L
[J]. NATURE METHODS, 2005, 2 (04) : 305 - 311
[4] Synthesis of conducting polyelectrolyte complexes of polyaniline and poly(2-acrylamido-3-methyl-1-propanesulfonic acid) catalyzed by pH-stable palm tree peroxidase
Caramyshev, AV
Evtushenko, EG
Ivanov, VF
Barcel, AR
Roig, MG
Shnyrov, VL
van Huystee, RB
Kurochkin, IN
Vorobiev, AK
Sakharov, IY
[J]. BIOMACROMOLECULES, 2005, 6 (03) : 1360 - 1366
[5] PNA HYBRIDIZES TO COMPLEMENTARY OLIGONUCLEOTIDES OBEYING THE WATSON-CRICK HYDROGEN-BONDING RULES
EGHOLM, M
BUCHARDT, O
CHRISTENSEN, L
BEHRENS, C
FREIER, SM
DRIVER, DA
BERG, RH
KIM, SK
NORDEN, B
NIELSEN, PE
[J]. NATURE, 1993, 365 (6446) : 566 - 568
[6] F Allen J Bard L.R., 2001, Electrochemical Methods: Fundamentals and Applications
[7] Detection of microRNAs using electrocatalytic nanoparticle tags
Gao, ZQ
Yang, ZC
[J]. ANALYTICAL CHEMISTRY, 2006, 78 (05) : 1470 - 1477
[8] SNP detection using peptide nucleic acid probes and conjugated polymers: Applications in neurodegenerative disease identification
Gaylord, BS
Massie, MR
Feinstein, SC
Bazan, GC
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (01) : 34 - 39
[9] DNA hybridization detection with water-soluble conjugated polymers and chromophore-labeled single-stranded DNA
Gaylord, BS
Heeger, AJ
Bazan, GC
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (04) : 896 - 900
[10] A formula for thermal stability (Tm) prediction of PNA/DNA duplexes
Giesen, U
Kleider, W
Berding, C
Geiger, A
Orum, H
Nielsen, PE
[J]. NUCLEIC ACIDS RESEARCH, 1998, 26 (21) : 5004 - 5006

← 1 2 3 →