Functional immune response in Pecten maximus: Combined effects of a pathogen-associated molecular pattern and PAH exposure

被引:14
作者
Hannam, Marie L. [1 ]
Bamber, Shaw D. [2 ]
Galloway, Tamara S. [3 ]
Moody, A. John [1 ]
Jones, Malcolm B. [4 ]
机构
[1] Univ Plymouth, Ecotoxicol & Stress Biol Res Ctr, Sch Biomed & Biol Sci, Plymouth PL4 8AA, Devon, England
[2] IRIS Biomiljo, N-4070 Randaberg, Norway
[3] Univ Exeter, Sch Biosci, Hatherly Labs, Exeter EX4 4PS, Devon, England
[4] Univ Plymouth, Inst Marine, Plymouth PL4 8AA, Devon, England
关键词
Pecten maximus; Scallop; Immune function; Phagocytosis; Lipopolysaccharide; PAMP; Polycyclic aromatic hydrocarbon; Phenanthrene; POLYCYCLIC AROMATIC-HYDROCARBONS; MUSSEL MYTILUS-GALLOPROVINCIALIS; CRASSOSTREA-GIGAS; PACIFIC OYSTER; MYA-ARENARIA; BLUE MUSSEL; EDULIS; HEMOCYTES; SCALLOP; STRESS;
D O I
10.1016/j.fsi.2009.10.018
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Pathogen-associated molecular patterns (PAMPs) enable recognition of structures present in microorganisms such as lipopolysaccharides (LPS). LPS are an essential constituent of the outer membrane of Gram-negative bacteria, stimulating the innate immune system of invertebrates. Here, LPS from Escherichia coli (055:B5) were used to investigate the functional immune response of Pecten maximus after stimulation with a PAMP and to determine the combined effect of a phenanthrene exposure and LPS challenge. Organisms were exposed to 200 mu g l(-1) phenanthrene and after 7 d were injected with either physiological saline (injection controls) or LPS solution, and returned to their respective exposure tanks. Haemolymph was sampled from the scallops 48 h post-injection and immune function was assessed using a combination of cellular biological responses. The LPS challenge significantly altered the immune response in P. maximus with increased cell counts and phagocytic activity. An immunosuppressive effect of phenanthrene was also observed in this study; however, exposure to phenanthrene did not significantly impair the organism's ability to respond to a PAMP challenge. The overall level of phagocytosis and cytotoxic capability following the LPS challenge was lower in phenanthrene exposed scallops and may have consequences for disease resistance in this commercially-exploited species. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:249 / 252
页数:4
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