Identification and characterization of chicken circovirus from commercial broiler chickens in China

被引：9
作者：
Li, Gen [1 ,2 ]
Yuan, Shiyu [1 ]
Yan, Tianxing [1 ]
Shan, Hu [2 ]
Cheng, Ziqiang [1 ]
机构：
[1] Shandong Agr Univ, Coll Vet Med, 61 Daizong St, Tai An 271018, Shandong, Peoples R China
[2] Qingdao Agr Univ, Coll Vet Med, Qingdao, Shandong, Peoples R China
来源：
TRANSBOUNDARY AND EMERGING DISEASES | 2020年 / 67卷 / 01期
关键词：
acute gastroenteritis; chicken circovirus; VIRUS-INFECTION; DISEASE VIRUS; POLYMERASE; PCR;
D O I：
10.1111/tbed.13331
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Circoviruses are found in many species, including mammals, birds, lower vertebrates and invertebrates. To date, there are no reports of circovirus-induced diseases in chickens. In this study, we identified a new strain of chicken circovirus (CCV) by PacBio third-generation sequencing samples from chickens with acute gastroenteritis in a Shandong commercial broiler farm in China. The complete genome of CCV was verified by inverse PCR. Genomic analysis revealed that CCV codes two inverse open reading frames (ORFs), and a potential stem-loop structure was present at the 5 ' end with a structure typical of a circular virus. Phylogenetic tree analysis showed that CCV formed an independent branch between mammalian and avian circovirus, and homology analysis indicated that the homology of CCV with 21 other known circoviruses was less than 40%. Thus, this CCV strain represents a new species in the genus Circovirus. The infection rate of CCV in 12 chickens with diarrhoea was 100%, but no CCV was found in healthy chickens, thereby indicating that the novel CCV strain is highly associated with acute infectious gastroenteritis in chickens. The emergence of a novel CCV in commercial broiler chickens is highly concerning for the broiler industry.
引用
收藏
页码：6 / 10
页数：5
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