Simultaneous monitoring of inorganic cations, amines and amino acids in human sweat by capillary electrophoresis

被引:54
|
作者
Hirokawa, Takeshi
Okamoto, Hikaru
Gosyo, Yoshinori
Tsuda, Takao
Timerbaev, Andrei R.
机构
[1] Hiroshima Univ, Grad Sch Engn, Dept Appl Chem, Higashihiroshima 7398527, Japan
[2] Nagoya IKOU RENKEI Incubator, PicoDevice Co, Chikusa Ku, Nagoya, Aichi 4640858, Japan
[3] Russian Acad Sci, VI Vernadskii Inst Geochem & Analyt Chem, Moscow 119991, Russia
[4] Univ Vienna, Inst Organ Chem, A-1090 Vienna, Austria
关键词
human sweat; capillary electrophoresis; sampling; cations; amino acids; amines;
D O I
10.1016/j.aca.2006.07.077
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The determination of cationic constituents of sweat is widely recognized as a difficult analytical task due to its complex composition and minute sample volumes available for the individual analysis. Capillary electrophoresis (CE) has been evaluated as a simple routine method to measure sweat metal cations, biogenic amines, and amino acids using a sampling procedure previously developed in one of collaborative teams. The carrier electrolyte, which consisted of 10 mM 4-methylbenzylamine, 6.5 mM alpha-hydroxyisobutyric acid, and 2 mM 18-crown-6 at pH 4.25, allowed the separation of five cations (NH4+, K+, Ca2+, Na+, Mg2+) and four amino acids (ornithine, histidine, lysine, arginine) to be completed in about 13 min with a positive polarity of the applied voltage (30kV). By increasing the sample volume (due to employing hydrodynamic instead of hydrostatic injection mode), it was also possible to detect indirect UV signals of Zn2+,, diethanolamine, and trithanolamine. Sweat samples were collected from the fingers and forearms of three healthy male volunteers and analyzed by CE. A good repeatability and reproducibility of peak area responses based on five intraday and three inter-day assays (average %R.S.D. less than 3.5 and 2.5, respectively) were obtained. The limits of detection were in the range of 3.2-5.8 mu M for alkali and alkaline-earth cations (hydrostatic injection) and 0.27-0.79 mu M for other target analytes (hydrodynamic injection). The analytical results for particular analytes were found to vary, depending on the sampling spot and individual, but in general correspond well to clinical concentration ranges. (c) 2006 Elsevier B.V. All rights reserved.
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页码:83 / 88
页数:6
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