SILK FIBROIN NANOPARTICLES REVEAL EFFICIENT DELIVERY OF 5-FU IN A HT-29 COLORECTAL ADENOCARCINOMA MODEL IN VITRO

被引：17

作者：

Radu, Ionut Cristian ^{[1
]}

Hudita, Ariana ^{[2
]}

Zaharia, Catalin ^{[1
]}

Negrei, Carolina ^{[3
]}

Dragomiroiu, George Traian Alexandru Burcea ^{[4
]}

Popa, Daniela Elena ^{[4
]}

Costache, Marieta ^{[2
]}

Iovu, Horia ^{[1
]}

Georgescu, Mara ^{[7
]}

Ginghina, Octav ^{[5
,6
]}

Galateanu, Bianca ^{[2
]}

机构：

[1] Univ Politehn Bucuresti, Adv Polymer Mat Grp, Bucharest, Romania

[2] Univ Bucharest, Dept Biochem & Mol Biol, Bucharest, Romania

[3] Carol Davila Univ Med & Pharm, Fac Pharm, Dept Toxicol, Bucharest, Romania

[4] Carol Davila Univ Med & Pharm, Fac Pharm, Dept Drug Control, Bucharest, Romania

[5] Sf Ioan Clin Emergency Hosp, Dept Surg, Bucharest, Romania

[6] Carol Davila Univ Med & Pharm, Fac Dent Med, Dept 2, Bucharest, Romania

[7] Univ Agron Sci & Vet Med Bucharest, Fac Vet Med, 59 Marasti Blvd, Bucharest 011464, Romania

来源：

FARMACIA | 2021年 / 69卷 / 01期

关键词：

5-FU; silk fibroin; nanoparticles; HT-29 adenocarcinoma cells; drug delivery systems; DRUG-DELIVERY; NANOMEDICINE;

D O I：

10.31925/farmacia.2021.1.15

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The aim of the paper was to investigate the efficiency of 5-FU delivery from smart silk fibroin nanoparticles in a HT-29 colorectal adenocarcinoma model in vitro. Therefore, nanoparticles with various amounts of silk fibroin were obtained by nanoprecipitation method and characterized to evaluate the 5-FU encapsulation efficiency and release profile. All nanoparticles showed a good entrapment of the drug and were capable to release 5 -FU in a polymer concentration dependent manner. The in vitro biological evaluation revealed an excellent biocompatibility of the pristine silk fibroin nanocarriers, while 5-FU loaded nanoparticles proved to be highly cytotoxic and dramatically decreased the HT-29 cellular viability and proliferation potential. More, the 5-FU silk fibroin nanoparticles induced alterations in the HT-29 cellular morphology and inhibited the formation of compact cellular clusters.

引用

页码：113 / 122

页数：10

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