New aqueous two-phase system based on cashew-nut tree gum and poly(ethylene glycol)

被引：28
|
作者：
Sarubbo, LA
Oliveira, LA
Porto, ALF
Duarte, HS
Carneiro-Leao, AMA
Lima, JL
Campos-Takaki, GM
Tambourgi, EB
机构：
[1] Univ Estadual Campinas, DESQ, FEQ, BR-13081 Campinas, Brazil
[2] Univ Fed Pernambuco, LAb Imunopatol Keizo Asami, Recife, PE, Brazil
[3] Univ Fed Rural Pernambuco, Dept Morfol & Fisiol Anim, Recife, PE, Brazil
[4] Univ Catolica Pernambuco, Dept Quim, Recife, PE, Brazil
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2000年 / 743卷 / 1-2期
基金：
巴西圣保罗研究基金会;
关键词：
aqueous two-phase systems; cashnew-nut tree gum; poly(ethylene glycol);
D O I：
10.1016/S0378-4347(99)00516-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The characterisation of a new system based on cashew-nut tree gum, a branched acidic heteropolysaccharide found in Brazil, and poly(ethylene glycol) (PEG) was studied. Phase diagrams are provided for the PEG-cashew-nut tree gum system. The influence of PEG molecular mass, tie-line length and pH on bovine serum albumin (BSA) partition was investigated. Protein partition coefficient was little influenced by changing PEG molecular mass. Increasing the tie-line length decreased the partition. Increasing the pH also raised the BSA partition coefficient. It is shown that systems formed by PEG and cashew-nut tree gum may be considered as an interesting alternative for use in protein purification. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：79 / 84
页数：6
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