New aqueous two-phase system based on cashew-nut tree gum and poly(ethylene glycol)

被引：28

作者：

Sarubbo, LA

Oliveira, LA

Porto, ALF

Duarte, HS

Carneiro-Leao, AMA

Lima, JL

Campos-Takaki, GM

Tambourgi, EB

机构：

[1] Univ Estadual Campinas, DESQ, FEQ, BR-13081 Campinas, Brazil

[2] Univ Fed Pernambuco, LAb Imunopatol Keizo Asami, Recife, PE, Brazil

[3] Univ Fed Rural Pernambuco, Dept Morfol & Fisiol Anim, Recife, PE, Brazil

[4] Univ Catolica Pernambuco, Dept Quim, Recife, PE, Brazil

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2000年 / 743卷 / 1-2期

基金：

巴西圣保罗研究基金会;

关键词：

aqueous two-phase systems; cashnew-nut tree gum; poly(ethylene glycol);

D O I：

10.1016/S0378-4347(99)00516-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The characterisation of a new system based on cashew-nut tree gum, a branched acidic heteropolysaccharide found in Brazil, and poly(ethylene glycol) (PEG) was studied. Phase diagrams are provided for the PEG-cashew-nut tree gum system. The influence of PEG molecular mass, tie-line length and pH on bovine serum albumin (BSA) partition was investigated. Protein partition coefficient was little influenced by changing PEG molecular mass. Increasing the tie-line length decreased the partition. Increasing the pH also raised the BSA partition coefficient. It is shown that systems formed by PEG and cashew-nut tree gum may be considered as an interesting alternative for use in protein purification. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：79 / 84

页数：6

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