Association between histopathological features of dysplasia in oral leukoplakia and loss of heterozygosity

被引：21
作者：
Fonseca-Silva, Thiago [1 ]
Diniz, Marina G. [1 ]
de Sousa, Silvia F. [1 ]
Gomez, Ricardo S. [1 ]
Gomes, Carolina C. [2 ]
机构：
[1] Univ Fed Minas Gerais, Dept Oral Surg & Pathol, Sch Dent, Inst Biol Sci, BR-31270901 Belo Horizonte, MG, Brazil
[2] Univ Fed Minas Gerais, Inst Biol Sci, Dept Pathol, BR-31270901 Belo Horizonte, MG, Brazil
来源：
HISTOPATHOLOGY | 2016年 / 68卷 / 03期
关键词：
dysplasia grading; loss of heterozygosity; molecular alterations; oral cancer; potentially malignant oral disorders; POTENTIALLY MALIGNANT DISORDERS; PREDICTIVE-VALUE; CLASSIFICATION; HEAD;
D O I：
10.1111/his.12746
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
AimsOral leukoplakia (OL) dysplasia is graded on the basis of architectural and cytological features, and grade does not correlate well with malignant transformation. Loss of heterozygosity (LOH) profiles have been validated as risk predictors of OL malignant transformation. We aimed to assess whether the histological parameters used to grade dysplasia show different LOH profiles. Methods and resultsAreas of epithelial dysplasia of 29 OL samples were microdissected, and LOH was assessed by use of a panel of 11 microsatellite markers located on chromosomes 3, 9, 11, and 17. Dysplasia was graded, and the cytological and architectural parameters were scored. Dysplasia was graded as mild in 18 samples, moderate in nine, and severe in two. The moderate/severe dysplasias and the mild dysplasias did not show different frequencies of allelic loss. Irregular epithelial stratification was associated with LOH at marker D3S1234 (3p14.2). In addition, the presence of drop-shaped rete ridges and premature keratinization in single cells showed associations with LOH at D9S162 (9p22) and P53 (17p13.1), respectively. ConclusionsWe provide evidence that architectural and cellular changes in OL have different LOH patterns.
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页码：456 / 460
页数：5
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