Preparation and maintenance of SCID-hu mice for HIV research

被引：9
作者：
Bristol, GC
Gao, LY
Zack, JA
机构：
[1] UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,DIV HEMATOL ONCOL,LOS ANGELES,CA 90095
[2] JONSSON COMPREHENS CANC CTR,LOS ANGELES,CA 90095
来源：
METHODS-A COMPANION TO METHODS IN ENZYMOLOGY | 1997年 / 12卷 / 04期
关键词：
D O I：
10.1006/meth.1997.0488
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The SCID-hu mouse bearing a functional human thymic implant can be easily infected with HIV. Infection results in virus replication and relatively rapid depletion of CD4(+) human thymocytes, resulting in a pathologic profile similar to that seen in the thymus of HIV-infected humans. The use of the SCID-hu model for HIV research requires protection of the animals from opportunistic infections and protection of the operators from human pathogens. This discussion describes reliable methods of animal care and surgical procedures to meet these needs. (C) 1997 Academic Press.
引用
收藏
页码：343 / 347
页数：5
相关论文
共 19 条
[1]  
AKKINA RK, 1994, BLOOD, V84, P1393
[2]   Replication and pathogenicity of human immunodeficiency virus type 1 accessory gene mutants in SCID-hu mice [J].
Aldrovandi, GM ;
Zack, JA .
JOURNAL OF VIROLOGY, 1996, 70 (03) :1505-1511
[3]   THE SCID-HU MOUSE AS A MODEL FOR HIV-1 INFECTION [J].
ALDROVANDI, GM ;
FEUER, G ;
GAO, LY ;
JAMIESON, B ;
KRISTEVA, M ;
CHEN, ISY ;
ZACK, JA .
NATURE, 1993, 363 (6431) :732-736
[4]  
*BIOS MICR BIOM LA, 01702300167 BIOS MIC
[5]   DEVELOPMENT OF A HUMAN THYMIC ORGAN-CULTURE MODEL FOR THE STUDY OF HIV PATHOGENESIS [J].
BONYHADI, ML ;
SU, LS ;
AUTEN, J ;
MCCUNE, JM ;
KANESHIMA, H .
AIDS RESEARCH AND HUMAN RETROVIRUSES, 1995, 11 (09) :1073-1080
[6]   A SEVERE COMBINED IMMUNODEFICIENCY MUTATION IN THE MOUSE [J].
BOSMA, GC ;
CUSTER, RP ;
BOSMA, MJ .
NATURE, 1983, 301 (5900) :527-530
[7]  
Centers for Disease Control (CDC), 1988, MMWR Suppl, V37, P1
[8]   MODELING HUMAN RETROVIRAL PATHOGENESIS AND ANTIRETROVIRAL GENE-THERAPY IN THE SCID MOUSE [J].
CHEN, ISY ;
ZACK, JA .
RESEARCH IN IMMUNOLOGY, 1994, 145 (05) :385-392
[9]  
FEUER G, 1993, BLOOD, V82, P722
[10]   IN-VIVO PATHOGENIC PROPERTIES OF 2 CLONAL HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ISOLATES [J].
JAMIESON, BD ;
PANG, S ;
ALDROVANDI, GM ;
ZHA, JL ;
ZACK, JA .
JOURNAL OF VIROLOGY, 1995, 69 (10) :6259-6264
12