Identification and Quantification of Glucosinolates in Kimchi by Liquid Chromatography-Electrospray Tandem Mass Spectrometry

A novel and simple method for detecting five glucosinolates (glucoalyssin, gluconapin, glucobrassicanapin, glucobrassicin, and 4-methoxyglucobrassicin) in kimchi was developed using liquid chromatography-electrospray tandem mass spectrometry (LCMS/ MS). The chromatographic peaks of the five glucosinolateswere successfully identified by comparing their retention times, mass spectra. The mobile phase was composed of A (acetonitrile) and B (water). As for glucosinolate, the relative quantities were found through sinigrin, and five different compounds that have not been previously discovered in kimchi were observed. Monitoring was carried out on the glucosinolate in 20 kimchis distributed in markets, and this study examined the various quality and quantity compositions of the five components. The glucoalyssin content ranged from 0.00 to 7.07 mu mol/g of day weight (DW), with an average content of 0.86 mu mol/g of DW, whereas the gluconapin content ranged from 0.00 to 5.85 mu mol/g of DW, with an average of 1.17 mu mol/g of DW. The content of glucobrassicanapin varied between 0.00 and 11.87 mu mol/g of DW (average = 3.03 mu mol/g of DW), whereas that of glucobrassicin varied between 0.00 and 0.42 mu mol/g of DW (average = 0.06 mu mol/g of DW). The 4methoxyglucobrassicin content ranged from 0.12 to 9.36 mu mol/g of DW (average = 3.52 mu mol/g of DW). A comparison of the contents revealed that, in most cases, the content of 4-methoxyglucobrassicin was the highest.