Fabrication of multi-biofunctional gelatin-based electrospun fibrous scaffolds for enhancement of osteogenesis of mesenchymal stem cells

被引：37

作者：

Lin, Wei-Han ^{[1
]}

Yu, Jiashing ^{[1
]}

Chen, Guoping ^{[2
]}

Tsai, Wei-Bor ^{[1
]}

机构：

[1] Natl Taiwan Univ, Dept Chem Engn, Taipei 106, Taiwan

[2] Natl Inst Mat Sci, Tissue Regenerat Mat Unit, Int Ctr Mat Nanoarchitecton MANA, Tsukuba, Ibaraki 3050044, Japan

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2016年 / 138卷

关键词：

Electrospun fibers; Gelatin; Osteogenesis; RGD; Hydroxyapatite; Bone morphogenetic protein-2; BONE REGENERATION; COMPOSITE NANOFIBERS; CALCIUM-PHOSPHATE; CROSS-LINKING; IN-VIVO; HYDROXYAPATITE; DIFFERENTIATION; RGD; ACID); IMMOBILIZATION;

D O I：

10.1016/j.colsurfb.2015.11.017

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Biofunctional scaffolds that support the adhesion, proliferation, and osteo-differentiation of mesenchymal stem cells (MSCs) are critical for bone tissue engineering. In this study, a simple in situ UV-crosslinking strategy was utilized to fabricate gelatin electrospun fibrous (GEF) scaffolds with multiple biosignals, including cell adhesive Arg-Gly-Asp (RGD) peptide, osteo-conductive hydroxyapatite (HAp) nanoparticles, and osteo-inductive bone morphogenic protein-2 (BMP-2). The adhesion and proliferation of MSCs on the GEF scaffolds were improved by the incorporation of RGD. Meanwhile, the incorporation of HAp and BMP-2 enhanced osteo-differentiation of MSCs. The three incorporated bio-factors exert a synergistic effect on osteogenesis of MSCs in the GEF scaffolds. This strategy of incorporating multiple biomolecules could be used to fabricate crosslinked electrospun scaffolds of natural polymers for tissue-engineering applications. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：26 / 31

页数：6

共 47 条

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