The application of RAPD markers in diversity detection and variety identification of Porphyra

被引：0

作者：

Jia, JH ^{[1
]}

Wang, P ^{[1
]}

Jin, DM ^{[1
]}

Qu, XP ^{[1
]}

Wang, Q ^{[1
]}

Li, CY ^{[1
]}

Weng, ML ^{[1
]}

Wang, B ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Genet, Beijing 100101, Peoples R China

来源：

ACTA BOTANICA SINICA | 2000年 / 42卷 / 04期

关键词：

RAPD; genetic diversity; cluster analysis; Porphyra; germplasm identification;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RAPD (random amplified polymorphic DNA) markers were generated from filaments of 15 Porphyra lines representing four important groups, P. yezoensis, P. haitanensis, P. katadai var. hemiphylla and P. oligospermatangia. Among the total 69 fragments generated by 6 selected primers (among 50 primers), 67 appeared to be polymorphic (97.1%). Cluster analysis based on the RAPD results was performed. The 15 Porphyra lines were divided into 3 groups. This result was consistent with that from taxonomy analysis. A DNA fingerprinting based on 8 bands amplified with OPN-02 and OPJ-18 was constructed and might be used in Porphyra variety identification. Five specific RAPD fragments of 5 Porhyra lines were isolated and cloned into pGEM-T easy vector. These five RAPD fragments may be useful in germplasm identification and property protection of Porphyra.

引用

页码：403 / 407

页数：5

共 7 条

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