Over-expression of CHAF1A promotes cell proliferation and apoptosis resistance in glioblastoma cells via AKT/FOX03a/Bim pathway

被引：33

作者：

Peng, Honghai ^{[1
]}

Du, Bin ^{[1
]}

Jiang, Huili ^{[2
]}

Gao, Jun ^{[1
]}

机构：

[1] Shandong Univ, Jinan Cent Hosp, Dept Neurosurg, Jinan 250013, Shandong, Peoples R China

[2] Shandong Univ, Jinan Cent Hosp, Friendship Nephrol & Blood Purificat Ctr, Jinan 250013, Shandong, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2016年 / 469卷 / 04期

关键词：

Glioblastoma; CHAFIA; CRISPR/CAS9; Proliferation; FOX03a; CANCER; FOXO3A; DIFFERENTIATION;

D O I：

10.1016/j.bbrc.2015.12.111

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromatinassembly factor 1 subunit A (CHAF1A) has been reported to be involved in several human diseases including cancer. However, the biological and clinical significance of CHAF1A in glioblastoma progression remains largely unknown. In this study, we found that up-regulation of CHAF1A happens frequently in glioblastoma tissues and is associated with glioblastoma prognosis. Knockout of CHAF1A by CRISPR/CAS9 technology induce G1 phase arrest and apoptosis in glioblastoma cell U251 and U87. In addition, inhibition of CHAF1A influenced the signal transduction of the AKT/FOX03a/Bim axis, which is required for glioblastoma cell proliferation. Taken together, these results show that CHAF1A contributes to the proliferation of glioblastoma cells and may be developed as a de novo drug target and prognosis biomarker of glioblastoma. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：1111 / 1116

页数：6

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