Transcriptional inhibition of feline immunodeficiency virus by alpha-amanitin

被引：1

作者：

Tanabe, Taishi ^{[1
]}

Fukuda, Yurina ^{[1
]}

Kawashima, Kazuhiko ^{[2
]}

Yamamoto, Satomi ^{[1
]}

Kashimoto, Takashige ^{[3
]}

Sato, Hisaaki ^{[1
]}

机构：

[1] Kitasato Univ, Sch Vet Med, Lab Vet Microbiol, Towada, Aomori 0348628, Japan

[2] Aino Anim Hosp, Takao 85-5, Fukuroi, Shizuoka 4370023, Japan

[3] Kitasato Univ, Sch Vet Med, Lab Vet Publ Hlth, Towada, Aomori 0348628, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2021年 / 83卷 / 01期

关键词：

amanitin; anti-retroviral drug; feline immunodeficiency virus (FIV); therapy; TRANSACTIVATOR GENE; LYMPHOTROPIC VIRUS; REPLICATION; TOXICITY; CATS; ORF2; PCR;

D O I：

10.1292/jvms.20-0182

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Alpha-amanitin, one of the a matoxins in egg amanita, has a cyclic peptide structure, and was reported as having antiviral activity against several viruses. We investigated whether alpha-amanitin has antiviral activity against feline immunodeficiency virus (FIV). FL-4 cells persistently infected with FIV Petaluma were cultured with alpha-amanitin. Reverse transcriptase (RT) activity in the supernatant of FL-4 cells was significantly inhibited by alpha-amanitin. In addition, the production of FIV core protein in FL-4 cells was inhibited by alpha-amanitin when analyzed by western blotting. Furthermore, alpha-amanitin inhibited the transcription of FIV in real-time RT-PCR. These data suggested that alpha-amanitin showed anti-FIV activity by inhibiting the RNA transcription level.

引用

页码：158 / 161

页数：4

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