Transcriptional inhibition of feline immunodeficiency virus by alpha-amanitin

被引：1
作者：
Tanabe, Taishi [1 ]
Fukuda, Yurina [1 ]
Kawashima, Kazuhiko [2 ]
Yamamoto, Satomi [1 ]
Kashimoto, Takashige [3 ]
Sato, Hisaaki [1 ]
机构：
[1] Kitasato Univ, Sch Vet Med, Lab Vet Microbiol, Towada, Aomori 0348628, Japan
[2] Aino Anim Hosp, Takao 85-5, Fukuroi, Shizuoka 4370023, Japan
[3] Kitasato Univ, Sch Vet Med, Lab Vet Publ Hlth, Towada, Aomori 0348628, Japan
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2021年 / 83卷 / 01期
关键词：
amanitin; anti-retroviral drug; feline immunodeficiency virus (FIV); therapy; TRANSACTIVATOR GENE; LYMPHOTROPIC VIRUS; REPLICATION; TOXICITY; CATS; ORF2; PCR;
D O I：
10.1292/jvms.20-0182
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Alpha-amanitin, one of the a matoxins in egg amanita, has a cyclic peptide structure, and was reported as having antiviral activity against several viruses. We investigated whether alpha-amanitin has antiviral activity against feline immunodeficiency virus (FIV). FL-4 cells persistently infected with FIV Petaluma were cultured with alpha-amanitin. Reverse transcriptase (RT) activity in the supernatant of FL-4 cells was significantly inhibited by alpha-amanitin. In addition, the production of FIV core protein in FL-4 cells was inhibited by alpha-amanitin when analyzed by western blotting. Furthermore, alpha-amanitin inhibited the transcription of FIV in real-time RT-PCR. These data suggested that alpha-amanitin showed anti-FIV activity by inhibiting the RNA transcription level.
引用
收藏
页码：158 / 161
页数：4
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