An integrated analysis based on transcriptome and proteome reveals deastringency-related genes in CPCNA persimmon

被引：30
作者：
Chen, Wenxing [1 ]
Xiong, Yalou [1 ]
Xu, Liqing [1 ]
Zhang, Qinglin [1 ]
Luo, Zhengrong [1 ,2 ]
机构：
[1] Huazhong Agr Univ, Key Lab Hort Plant Biol, Wuhan 430070, Hubei, Peoples R China
[2] Huanggang Normal Univ, Hubei Collaborat Innovat Ctr Characterist Resourc, Huanggang 438000, Hubei, Peoples R China
来源：
SCIENTIFIC REPORTS | 2017年 / 7卷
关键词：
CARBON-DIOXIDE TREATMENT; DIOSPYROS-KAKI THUNB; RNA-SEQ DATA; PROANTHOCYANIDIN BIOSYNTHESIS; POLLINATION-CONSTANT; ASTRINGENCY REMOVAL; FRUIT; EXPRESSION; ACETALDEHYDE; BIOCHEMISTRY;
D O I：
10.1038/srep44671
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Persimmon fruits accumulate a large amount of proanthocyanidins (PAs) during development. PAs cause a dry or puckering sensation due to its astringency. Pollination constant and non-astringent (PCNA) persimmon fruits can lose astringency during fruit ripening. However, little is known about the mechanism of natural de-astringency of Chinese PCNA (CPCNA). To gain insight into the molecular events of CPCNA natural de-astringency, we used mRNA-seq and iTRAQ-based quantitative proteomic analysis to measure changes in genes and proteins expression at two key stages of natural astringency removal (i.e. 10 and 20 weeks after bloom) and water-treated (i.e. 40 degrees C.12h) de-astringency fruits. Our analyses show that the three predominantly process in CPCNA de-astringency: (1) water treatment strongly up-regulates glycolysis/acetaldehyde metabolism, (2) expression of genes/proteins involved in PA biosynthetic pathway was remarkably reduced in natural and water-treated de-astringency, (3) sugar metabolism and ethylene related pathway were quite abundant in natural de-astringency. We also found ethylene-related TFs were quite abundant in natural de-astringency, followed by WRKY and NAC transcription factors. These results provide an initial understanding of the predominantly biological processes underlying the natural de-astringency and "coagulation effect" in CPCNA.
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页数：18
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