Laser-scribed Graphene Electrodes as an Electrochemical Immunosensing Platform for Cancer Biomarker 'eIF3d'

被引：10

作者：

Balaban, Simge ^{[1
]}

Beduk, Tutku ^{[2
]}

Durmus, Ceren ^{[1
]}

Aydindogan, Eda ^{[1
]}

Salama, Khaled Nabil ^{[2
]}

Timur, Suna ^{[1
,3
]}

机构：

[1] Ege Univ, Dept Biochem, Fac Sci, TR-35100 Izmir, Turkey

[2] King Abdullah Univ Sci & Technol KAUST, Comp Elect & Math Sci & Engn Div, Sensors Lab, Adv Membranes & Porous Mat Ctr, Thuwal 239556900, Saudi Arabia

[3] Ege Univ, Res & Applicat Ctr, Cent Res Testing & Anal Lab, TR-35100 Izmir, Turkey

来源：

ELECTROANALYSIS | 2021年 / 33卷 / 04期

关键词：

Laser-scribed graphene electrodes; Cancer; Biosensors; Affinity-based sensors; Biomarkers; EIF3D; LABEL-FREE DETECTION; MESOPOROUS CARBON; BIOSENSORS; PROLIFERATION; KNOCKDOWN; PROTEIN; FILMS; XPS;

D O I：

10.1002/elan.202060482

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

eIF3d is a protein biomarker which has a potential for the diagnosis of various cancers. Herein, a bio-platform was constructed for eIF3d sensing by using LSG and surface functionalization with anti eIF3d antibody via EDC/NHS chemistry. Following the surface modifications, XPS and several electrochemical methods were used. Difference in the signals were related to biomarker amounts between 75-500 ng/mL. LOD was calculated as 50.4 ng/mL. Selectivity of biosensor was tested by using of various interference molecules. EIF3d was also successfully detected in synthetic biological samples. Thus, to the best of our knowledge, this study is one of the rare studies on use of LSGs in immunosensor studies.

引用

页码：1072 / 1080

页数：9

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