Methanogenic population dynamics during startup of a full-scale anaerobic sequencing batch reactor treating swine waste

被引:212
作者
Angenent, LT
Sung, SW
Raskin, L
机构
[1] Univ Illinois, Dept Civil & Environm Engn, Newmark Civil Engn Lab 3221, Urbana, IL 61801 USA
[2] Iowa State Univ, Dept Civil & Construct Engn, Ames, IA USA
关键词
ammonia; anaerobic; anaerobic sequencing batch reactor; methanogens; swine waste; 16S rRNA;
D O I
10.1016/S0043-1354(02)00199-9
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Changes in methanogenic population levels were followed during startup of a full-scale, farm-based anaerobic sequencing batch reactor (ASBR) and these changes were linked to operational and performance data. The ASBR was inoculated with anaerobic digester sludge from a municipal wastewater treatment facility. During an acclimation period of approximately 3 months, the ASBR content was diluted to maintain a total ammonia-N level of approximately 2000 mg l(-1). After this acclimation period, the volatile solids loading rate was increased to its design value of 1.7 g l(-1) day(-1) with a 15-day hydraulic retention time, which increased the total ammonia-N level in the ASBR to approximately 3600 mg l(-1). The 16S ribosomal RNA (rRNA) levels of the acetate-utilizing methanogens of the genus Methanosarcina decreased from 3.8% to 1.2% (expressed as a percentage of the total 16S rRNA levels) during this period, while the 16S rRNA levels of Methanosaeta concilii remained low (below 2.2%). Methane production and reactor performance were not affected as the 16S rRNA levels of the hydrogen-utilizing methanogens of the order Methanomicrobiales increased from 2.3% to 7.0%. Hence, it is likely that during operation with high ammonia levels, the major route of methane production is through a syntrophic relationship between acetate-oxidizing bacteria and hydrogen-utilizing methanogens. Anaerobic digestion at total ammonia-N levels exceeding 3500 mg l(-1) was sustainable apparently due to the acclimation of hydrogen-utilizing methanogens to high ammonia levels. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:4648 / 4654
页数:7
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