Combining Carbon Nanotubes and Chitosan for the Vectorization of Methotrexate to Lung Cancer Cells

被引：44

作者：

Cirillo, Giuseppe ^{[1
,2
]}

Vittorio, Orazio ^{[3
,4
,5
]}

Kunhardt, David ^{[1
]}

Valli, Emanuele ^{[3
,5
]}

Voli, Florida ^{[3
]}

Farfalla, Annafranca ^{[2
]}

Curcio, Manuela ^{[2
]}

Spizzirri, Umile Gianfranco ^{[2
]}

Hampel, Silke ^{[1
]}

机构：

[1] Leibniz Inst Solid State & Mat Res Dresden, D-01069 Dresden, Germany

[2] Univ Calabria, Dept Pharm Hlth & Nutr Sci Univ, I-87036 Arcavacata Di Rende, CS, Italy

[3] UNSW Sydney, Childrens Canc Inst, Lowy Canc Res Ctr, Sydney, NSW 2031, Australia

[4] UNSW Sydney, Australian Ctr NanoMed, Arc Ctr Excellence Convergent BioNano Sci & Techn, Sydney, NSW 2052, Australia

[5] UNSW Sydney, Sch Womens & Childrens Hlth, Fac Med, Sydney, NSW 2052, Australia

来源：

MATERIALS | 2019年 / 12卷 / 18期

关键词：

multi-walled carbon nanotubes; nanohybrids; methotrexate; pH responsivity; lung cancer; BIOMEDICAL APPLICATIONS; NANOPARTICLES; THERAPY; CHEMOTHERAPY; RELEASE; FUNCTIONALIZATION; NANOCOMPOSITES; NANOMATERIALS; CYTOTOXICITY; NANOCARRIERS;

D O I：

10.3390/ma12182889

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A hybrid system composed of multi-walled carbon nanotubes coated with chitosan was proposed as a pH-responsive carrier for the vectorization of methotrexate to lung cancer. The effective coating of the carbon nanostructure by chitosan, quantified (20% by weight) by thermogravimetric analysis, was assessed by combined scanning and transmission electron microscopy, and X-ray photoelectron spectroscopy (N1s signal), respectively. Furthermore, Raman spectroscopy was used to characterize the interaction between polysaccharide and carbon counterparts. Methotrexate was physically loaded onto the nanohybrid and the release profiles showed a pH-responsive behavior with higher and faster release in acidic (pH 5.0) vs. neutral (pH 7.4) environments. Empty nanoparticles were found to be highly biocompatible in either healthy (MRC-5) or cancerous (H1299) cells, with the nanocarrier being effective in reducing the drug toxicity on MRC-5 while enhancing the anticancer activity on H1299.

引用

页数：14

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