Direct electrochemistry and bioelectrocatalysis of myoglobin at a carbon nanotube-modified electrode

被引：24

作者：

Lue Ya-Fen ^{[1
]}

Yin Ya-Jing ^{[1
]}

Wu Ping ^{[1
]}

Cai Chen-Xin ^{[1
]}

机构：

[1] Nanjing Normal Univ, Coll Chem & Environm Sci, Jiangsu Key Lab Mol & Med Biotechnol, Nanjing 210097, Peoples R China

来源：

ACTA PHYSICO-CHIMICA SINICA | 2007年 / 23卷 / 01期

基金：

中国国家自然科学基金;

关键词：

bioelectrocatalysis; direct electron transfer; carbon nanotube; hydrogen peroxide; myoglobin;

D O I：

10.1016/S1872-1508(07)60002-3

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Myoglobin (Mb) was immobilized onto the surface of carbon nanotube (CNT) and was characterized by AFM, XPS, UV-Vis, and FTIR spectroscopy. The promotion effect of CNT on direct electron transfer (DET) reaction of Mb was studied. Cyclic voltammetric results showed a pair of well-defined redox peaks, which corresponded to DET of Mb, with the formal potential (E-o') of (-0.343 +/- 0.001) V (vs SCE) in the phosphate buffer solution (PBS, pH 7.0). The electrochemical parameters, such as apparent heterogeneous electron transfer rate constant (k(s)) and E-o', were determined. The dependence of E-o' on solution pH indicated that DET reaction of Mb was coupled with proton transfer. The experimental results also demonstrated that the immobilized Mb retained its bioelectrocatalytic activity toward the reduction of H2O2 and O-2.

引用

页码：5 / 11

页数：7

共 48 条

[41]

Vecchio G, 1999, BIOTECHNOL BIOENG, V64, P545, DOI 10.1002/(SICI)1097-0290(19990905)64:5<545::AID-BIT4>3.0.CO

[42]

2-Y

[43] Carbon-nanotube based electrochemical biosensors: A review [J].

Wang, J .

ELECTROANALYSIS, 2005, 17 (01) :7-14

[44] Direct electrochemistry of cytochrome c at a glassy carbon electrode modified with single-wall carbon nanotubes [J].

Wang, JX ;

Li, MX ;

Shi, ZJ ;

Li, NQ ;

Gu, ZN .

ANALYTICAL CHEMISTRY, 2002, 74 (09) :1993-1997

[45] Direct electrochemistry of microperoxidase 11 using carbon nanotube modified electrodes [J].

Wang, MK ;

Shen, Y ;

Liu, Y ;

Wang, T ;

Zhao, F ;

Liu, BF ;

Dong, SJ .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2005, 578 (01) :121-127

[46] Direct electrochemistry of microperoxidase at Pt microelectrodes modified with carbon nanotubes [J].

Wang, MK ;

Zhao, F ;

Liu, Y ;

Dong, SJ .

BIOSENSORS & BIOELECTRONICS, 2005, 21 (01) :159-166

[47] Peroxidase activity of enzymes bound to the ends of single-wall carbon nanotube forest electrodes [J].

Yu, X ;

Chattopadhyay, D ;

Galeska, I ;

Papadimitrakopoulos, F ;

Rusling, JF .

ELECTROCHEMISTRY COMMUNICATIONS, 2003, 5 (05) :408-411

[48] Direct electrochemistry of cytochrome c on a multi-walled carbon nanotubes modified electrode and its electrocatalytic activity for the reduction of H2O2 [J].

Zhao, GC ;

Yin, ZZ ;

Zhang, L ;

Wei, XW .

ELECTROCHEMISTRY COMMUNICATIONS, 2005, 7 (03) :256-260

← 1 2 3 4 5 →