Effect of lipopolysaccharide on intranasal administration of liposomal Newcastle disease virus vaccine to SPF chickens

被引：33

作者：

Tseng, Li-Ping ^{[2
]}

Chiou, Chwei-Jang ^{[3
]}

Chen, Chien-Chung ^{[1
]}

Deng, Ming-Chung ^{[4
]}

Chung, Tze-Wen ^{[5
]}

Huang, Yi-You ^{[2
]}

Liu, Der-Zen ^{[1
]}

机构：

[1] Taipei Med Univ, Grad Inst Biomed Mat & Engn, Taipei, Taiwan

[2] Natl Taiwan Univ, Coll Med, Coll Engn, Grad Inst Biomed Engn, Taipei, Taiwan

[3] Taipei Med Univ, Coll Oral Med, Grad Inst Dent, Taipei, Taiwan

[4] Council Agr, Anim Hlth Res Inst, Dept Hog Cholera, Taipei, Taiwan

[5] Natl Yunlin Univ Sci & Technol, Dept Chem Engn, Yunlin, Taiwan

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2009年 / 131卷 / 3-4期

关键词：

Liposome; Adjuvant; Intranasal; Newcastle disease virus; s-IgA; LPS; IMMUNE-RESPONSE; ANTIBODY; RECEPTOR; IMMUNIZATION; RECOGNITION; ADJUVANTS; MOUSE; LPS;

D O I：

10.1016/j.vetimm.2009.04.009

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In order to potentiate the low immunogenicity of the inactivated Newcastle disease virus immunized into chickens by mucosal route, liposomes as a drug delivery system and LPS (lipopolysaccharide) as an immuno-stimulator were evaluated. Here, we report a new nasal delivery system of inactivated Newcastle disease virus (NDV) vaccine. The intranasal vaccine was based on different lipids to form MLV (multi-lamellar vehicles) liposomes. The liposomes had combined carrier and adjuvant activities, which induced strong systemic (serum) and local (lung and nasal) humoral responses in SPF (specific-pathogen-free) chickens, and provided protective immunity. PC-Lip (phosphatidylcholine-liposome) elicited significant mucosal secretary immunoglobulin A (s-IgA) levels (p < 0.05) in tracheal lavage fluid and serum IgG levels (p < 0.05). In response to virulent viral challenge, birds treated with PBS (phosphate buffered saline) as control group died, whereas 80% of chickens which received PC-Lip, PC-Lip-LPS, PS-Lip (phosphatidylserine-liposome), and PS-Lip-LPS survived. HAI titers were 1:2560 in the PS-Lip-LPS group and 1: 1280 in the PC-Lip, PC-Lip-LPS, and PS-Lip groups after two vaccinations. The results suggest that PC-Lip or PS-Lip might thus be suitable as a potential adjuvant for mucosal vaccination against NDV in chickens. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：285 / 289

页数：5

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