Evaluation of a triplex real-time PCR system to detect the plant-pathogenic molds Alternaria spp., Fusarium spp. and C. purpurea

被引：9

作者：

Grube, Sabrina ^{[1
,2
]}

Schoenling, Jutta ^{[3
]}

Prange, Alexander ^{[1
,2
,4
]}

机构：

[1] Niederrhein Univ Appl Sci, Microbiol & Food Hyg, D-41065 Monchengladbach, Germany

[2] Univ Witten Herdecke, Inst Microbiol & Virol, D-58448 Witten, Germany

[3] GEN IAL GmbH, D-53842 Troisdorf, Germany

[4] Louisiana State Univ, CAMD, Baton Rouge, LA 70806 USA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2015年 / 119卷

关键词：

Plant pathogen molds; Fungal contamination; Fusarium spp; Alternaria spp; C; purpurea; Real-time PCR; DNA;

D O I：

10.1016/j.mimet.2015.10.017

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This article describes the development of a triplex real-time PCR system for the simultaneous detection of three major plant-pathogenic mold genera (Alternaria spp., Fusarium spp. and the species Claviceps purpurea). The designed genus-specific primer-probe systems were validated for sensitivity, specificity and amplification in the presence of background DNA. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：180 / 188

页数：9

共 21 条

[1]

[Anonymous], FOOD MYCOLOGY MULTIF

[2]

Bartnicki-Garcia S, 1968, ANNU REV MICROBIOL, V2, P88

[3] ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases [J].

Bellemain, Eva ;

Carlsen, Tor ;

Brochmann, Christian ;

Coissac, Eric ;

Taberlet, Pierre ;

Kauserud, Havard .

BMC MICROBIOLOGY, 2010, 10

[4]

Chitarra G.S., 2007, FOOD MYCOLOGY MULTIF

[5] Moulds in food spoilage [J].

Filtenborg, O ;

Frisvad, JC ;

Thrane, U .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1996, 33 (01) :85-102

[6] Comparison of six DNA extraction methods for recovery of fungal DNA as assessed by quantitative PCR [J].

Fredricks, DN ;

Smith, C ;

Meier, A .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (10) :5122-5128

[7]

Geisen R, 2007, FOOD MYCOLOGY MULTIF

[8]

Geisen R, 2010, MOLEKULARBIOLOGISCHE, P193

[9] Strain-Dependent Variation in 18S Ribosomal DNA Copy Numbers in Aspergillus fumigatus [J].

Herrera, M. L. ;

Vallor, A. C. ;

Gelfond, J. A. ;

Patterson, T. F. ;

Wickes, B. L. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (05) :1325-1332

[10] Rapid preparation of Fusarium DNA from cereals for diagnostic PCR using sonification and an extraction kit [J].

Knoll, S ;

Mulfinger, S ;

Niessen, L ;

Vogel, RF .

PLANT PATHOLOGY, 2002, 51 (06) :728-734

← 1 2 3 →