Selection of optimal threshold is the most crucial issue in threshold-based segmentation. In case of color image, this task is become challenging, because conventional color image segmentation has computational complexity and also it suffers from lack of accuracy. Various techniques such as threshold based, region growing, edge detection, graph cut, pixel classification, neural network, active contour, gray level co-occurrence matrix are proposed so far for image segmentation in the literature. Out of them, threshold-based segmentation is popular for its simplicity. To address the problem of color image segmentation, we propose an enhanced version of metaheuristic optimization algorithm called Opposition based Symbiotic Organisms Search (OSOS) to solve multilevel image thresholding technique for color image segmentation by introducing opposition based learning concepts to accelerate the convergence rate and enhance the performance of standard symbiotic organisms search (SOS). The performance of the proposed OSOS based algorithm is investigated thoroughly and compared with some existing techniques like Cuckoo Search (CS), BAT algorithm (BAT), artificial bee colony (ABC) and particle swarm optimization (PSO). The comparison is made by applying the algorithm to a set of color images taken from a well-known benchmark dataset (Berkeley Segmentation Dataset (BSDS)) and some of the color images collected for the COCO dataset. It is observed from the results that the performance of the OSOS based algorithm is promising with respect to standards SOS and others in terms of the values of objective functions as well as the values of some well-defined quality metrics such as peak signal-to-noise ratio (PSNR), structure similarity index (SSIM) and feature similarity index (FSIM). The results of the proposed algorithm may encourage the scientists and engineers to apply it into pattern recognition problems. (C) 2019 Elsevier B.V. All rights reserved.