Factors affecting antigen uptake by human intestinal epithelial cell lines

被引:16
作者
So, ALP
Small, G
Sperber, K
Becker, K
Oei, E
Tyorkin, M
Mayer, L
机构
[1] Mt Sinai Med Ctr, Dept Microbiol, Div Clin Immunol, New York, NY 10029 USA
[2] Mt Sinai Med Ctr, Dept Med, New York, NY 10029 USA
[3] Mt Sinai Med Ctr, Dept Cell Biol, New York, NY 10029 USA
关键词
antigen uptake; immune complexes; cytokines;
D O I
10.1023/A:1005593717644
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
We assessed the role of size, solubility, and prophagocytic cytokines interferon-gamma (IFN-gamma), and granulocyte-macrophage colony stimulatory factor (GM-CSF) in antigen uptake and kinetics by intestinal epithelial cells using keyhole limpet hemocyanin and ovalbumin. Both fluoresceinated keyhole limpet hemocyanin (3000-7500 kDa) and fluoresceinated ovalbumin (45 kDa) were internalized by human colonic epithelial cell lines, with kinetics similar to those of fluoresceinated tetanus toroid, and there was decreased uptake of insoluble immune complexes and no enhancement in the uptake of soluble immune complexes. In addition, neither IFN-gamma nor GM-CSF altered the kinetics of uptake nor enhanced antigen internalization by the intestinal epithelial cell lines. These data suggest that regardless of the size of the soluble antigen, the presence of prophagocytic cytokines, or the formation of soluble immune complexes, fluid phase endocytosis of antigen by intestinal epithelial cells appears to be a relatively stable process.
引用
收藏
页码:1130 / 1137
页数:8
相关论文
共 34 条
  • [1] REOVIRUS-1 AND REOVIRUS-3 BIND AND INTERNALIZE AT THE APICAL SURFACE OF INTESTINAL EPITHELIAL-CELLS
    AMBLER, L
    MACKAY, M
    [J]. VIROLOGY, 1991, 184 (01) : 162 - 169
  • [2] IDENTIFICATION AND PARTIAL CHARACTERIZATION OF A RHESUS ROTAVIRUS BINDING GLYCOPROTEIN ON MURINE ENTEROCYTES
    BASS, DM
    MACKOW, ER
    GREENBERG, HB
    [J]. VIROLOGY, 1991, 183 (02) : 602 - 610
  • [3] BLAND PW, 1986, IMMUNOLOGY, V58, P1
  • [4] LIGHT AND ELECTRON MICROSCOPY OF INTESTINAL FERRITIN ABSORPTION . OBSERVATIONS IN SENSITIZED AND NONSENSITIZED HAMSTERS (MESOCRICETUS AURATUS)
    BOCKMAN, DE
    WINBORN, WB
    [J]. ANATOMICAL RECORD, 1966, 155 (04): : 603 - +
  • [5] COLEMAN DL, 1988, BLOOD, V72, P573
  • [6] EBERT EC, 1990, CLIN EXP IMMUNOL, V82, P81
  • [7] BACTERIAL ENTRY INTO EUKARYOTIC CELLS
    FALKOW, S
    [J]. CELL, 1991, 65 (07) : 1099 - 1102
  • [8] ASSOCIATION OF SUBEPITHELIAL DEPOSITION OF ACTIVATED COMPLEMENT AND IMMUNOGLOBULIN-G AND IMMUNOGLOBULIN-M RESPONSE TO GLUTEN IN CELIAC-DISEASE
    HALSTENSEN, TS
    HVATUM, M
    SCOTT, H
    FAUSA, O
    BRANDTZAEG, P
    [J]. GASTROENTEROLOGY, 1992, 102 (03) : 751 - 759
  • [9] LOCAL COMPLEMENT ACTIVATION IN INFLAMMATORY BOWEL-DISEASE
    HALSTENSEN, TS
    BRANDTZAEG, P
    [J]. IMMUNOLOGIC RESEARCH, 1991, 10 (3-4) : 485 - 492
  • [10] EPITHELIAL DEPOSITS OF IMMUNOGLOBULIN G1 AND ACTIVATED COMPLEMENT COLOCALISE WITH THE M(R) 40 KD PUTATIVE AUTOANTIGEN IN ULCERATIVE-COLITIS
    HALSTENSEN, TS
    DAS, KM
    BRANDTZAEG, P
    [J]. GUT, 1993, 34 (05) : 650 - 657