Impedimetric Detection of DNA Damage with the Sensor Based on Silver Nanoparticles and Neutral Red

被引：17

作者：

Kuzin, Yury ^{[1
]}

Porfireva, Anna ^{[1
]}

Stepanova, Veronika ^{[1
]}

Evtugyn, Vladimir ^{[2
]}

Stoikov, Ivan ^{[1
]}

Evtugyn, Gennady ^{[1
,3
]}

Hianik, Tibor ^{[3
,4
]}

机构：

[1] Kazan Fed Univ, AM Butlerov Chem Inst, Kazan 420008, Russia

[2] Kazan Fed Univ, Interdisciplinary Ctr Analyt Microscopy, Kazan 420008, Russia

[3] Kazan Fed Univ, OpenLab DNA Sensors, Kazan 420008, Russia

[4] Comenius Univ, Dept Nucl Phys & Biophys, Bratislava 84248, Slovakia

来源：

ELECTROANALYSIS | 2015年 / 27卷 / 12期

关键词：

DNA sensor; Silver nanoparticles; Neutral red; Oxidative DNA damage; Antioxidant capacity; TOTAL ANTIOXIDANT CAPACITY; METHYLENE-BLUE; ELECTROCHEMICAL BIOSENSOR; OXIDATIVE DAMAGE; PROTECTION; SYSTEM; FILMS; OXIDE;

D O I：

10.1002/elan.201500312

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Novel electrochemical DNA-sensor based on glassy carbon electrode (GCE) modified with Ag nanoparticles, Neutral red covalently attached to its surface and native DNA adsorbed on modifier coating was developed for the estimation of DNA damage on example of model system based on Fenton reagent. As was shown, the oxidation process resulted in synchronous increase of electron transfer resistance and capacitance measured by electrochemical impedance spectroscopy (EIS). The contribution of each sensor component on the signal was specified and sensitivity estimated against similar surface coatings. The shift of EIS parameters was found to be higher than that of similar biosensors reported. The DNA sensor was tested on the estimation of antioxidant capacity of green tea infusions again the results of coulometric titration with electrogenerated bromine.

引用

页码：2800 / 2808

页数：9

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