Impact of the chemicals, essential for the purification process of strict Fe-hydrogenase, on the corrosion of mild steel

被引:7
作者
Rouvre, Ingrid [1 ]
Gauquelin, Charles [2 ,3 ,4 ]
Meynial-Salles, Isabelle [2 ,3 ,4 ]
Basseguy, Regine [1 ]
机构
[1] Univ Fed Toulouse, Lab Genie Chim, CNRS, INP,UPS, 4 Allee Emile Monso,BP 84234, F-31432 Toulouse, France
[2] Univ Fed Toulouse, INSA, UPS, INP,LISBP, 135 Ave Rangueil, F-31077 Toulouse, France
[3] INRA, Ingn Syst Biol & Proc UMR792, F-31400 Toulouse, France
[4] CNRS, UMR5504, F-31400 Toulouse, France
关键词
Hydrogenase; Anaerobic corrosion; Dithiothreitol (DTT); Dithionite; Desthiobiotin; Mild steel; CONSTANT-PHASE-ELEMENT; 316L STAINLESS-STEEL; CLOSTRIDIUM-ACETOBUTYLICUM; STREP-TAG; LOCALIZED CORROSION; IMPEDANCE RESPONSE; PITTING CORROSION; ONLY HYDROGENASES; SULFATE; THIOSULFATE;
D O I
10.1016/j.bioelechem.2015.12.006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The influence of additional chemical molecules, necessary for the purification process of [Fe]-hydrogenase from Clostridium acetobutylicum, was studied on the anaerobic corrosion of mild steel. At the end of the purification process, the pure [Fe-Fe]-hydrogenase was recovered in a Tris-HCl medium containing three other chemicals at low concentration: DTT, dithionite and desthiobiotin. Firstly, mild steel coupons were exposed in parallel to a 0.1 M pH 7 Tris-HCl medium with or without pure hydrogenase. The results showed that hydrogenase and the additional molecules were in competition, and the electrochemical response could not be attributed solely to hydrogenase. Then, solutions with additional chemicals of different compositions were studied electrochemically. DTT polluted the electrochemical signal by increasing the E-oc by 35 mV 24 h after the injection of 300 mu L of control solutions with DTT, whereas it drastically decreased the corrosion rate by increasing the charge transfer resistance (R-ct 10 times the initial value). Thus, DTT was shown to have a strong antagonistic effect on corrosion and was removed from the purification process. An optimal composition of the medium was selected (0.5 mM dithionite, 7.5 mM desthiobiotin) that simultaneously allowed a high activity of hydrogenase and a lower impact on the electrochemical response for corrosion tests. (C) 2016 Elsevier B.V. All rights reserved.
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页码:9 / 23
页数:15
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