Diversity and distribution of Burkholderia cepacia complex in the rhizosphere of rice and maize

被引：34

作者：

Zhang, Lixin ^{[1
]}

Xie, Guanlin ^{[1
]}

机构：

[1] Zhejiang Univ, Inst Biotechnol, Hangzhou 310029, Peoples R China

来源：

FEMS MICROBIOLOGY LETTERS | 2007年 / 266卷 / 02期

关键词：

Burkholderia cepacia complex; diversity; distribution; rhizosphere; rice; maize;

D O I：

10.1111/j.1574-6968.2006.00530.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A survey of Burkholderia cepacia complex (Bcc) species was conducted in agricultural fields within Hangzhou, China. Out of the 251 bacterial isolates recovered on the selective media from the rhizosphere of rice and maize, 112 of them were assigned to Bcc by PCR assays. The species composition of the Bcc isolates was analyzed by a combination of recA-restriction fragment length polymorphism assays, species-specific PCR tests and recA gene sequencing. The results revealed that the majority belong to B. cepacia, Burkholderia cenocepacia recA lineage IIIB, Burkholderia vietnamiensis and Burkholderia pyrrocinia. Burkholderia cenocepacia and B. vietnamiensis dominated the rhizosphere of maize and rice, respectively, indicating that species composition and abundance of Bcc may vary dramatically in different crop rhizospheres. In addition, one isolate (R456) formed a single discrete cluster within the phylogenetic analysis of the Bcc recA gene, and it may belong to a new genomovar.

引用

页码：231 / 235

页数：5

共 29 条

[1] Multilocus sequence typing scheme that provides both species and strain differentiation for the Burkholderia cepacia complex [J].

Baldwin, A ;

Mahenthiralingam, E ;

Thickett, KM ;

Honeybourne, D ;

Maiden, MCJ ;

Govan, JR ;

Speert, DP ;

LiPuma, JJ ;

Vandamme, P ;

Dowson, CG .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (09) :4665-4673

[2] Burkholderia cepacia complex bacteria from clinical and environmental sources in Italy:: Genomovar status and distribution of traits related to virulence and transmissibility [J].

Bevivino, A ;

Dalmastri, C ;

Tabacchioni, S ;

Chiarini, L ;

Belli, ML ;

Piana, S ;

Materazzo, A ;

Vandamme, P ;

Manno, G .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (03) :846-851

[3]

BURBAGE DA, 1982, PHYTOPATHOLOGY, V72, P706

[4]

BURKHOLDER WH, 1950, PHYTOPATHOLOGY, V40, P115

[5] BURKHOLDERIA CEPACIA AND CYSTIC-FIBROSIS - DO NATURAL ENVIRONMENTS PRESENT A POTENTIAL HAZARD [J].

BUTLER, SL ;

DOHERTY, CJ ;

HUGHES, JE ;

NELSON, JW ;

GOVAN, JRW .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (04) :1001-1004

[6] Burkholderia cepacia complex species:: health hazards and biotechnological potential [J].

Chiarini, Luigi ;

Bevivino, Annamaria ;

Dalmastri, Claudia ;

Tabacchioni, Silvia ;

Visca, Paolo .

TRENDS IN MICROBIOLOGY, 2006, 14 (06) :277-286

[7] Burkholderia ambifaria sp nov., a novel member of the Burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates [J].

Coenye, T ;

Mahenthiralingam, E ;

Henry, D ;

LiPuma, JJ ;

Laevens, S ;

Gillis, M ;

Speert, DP ;

Vandamme, P .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :1481-1490

[8] Burkholderia cepacia genomovar VI, a new member of the Burkholderia cepacia complex isolated from cystic fibrosis patients [J].

Coenye, T ;

LiPuma, JJ ;

Henry, D ;

Hoste, B ;

Vandemeulebroecke, K ;

Gillis, M ;

Speert, DP ;

Vandamme, P .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :271-279

[9] Diversity and significance of Burkholderia species occupying diverse ecological niches [J].

Coenye, T ;

Vandamme, P .

ENVIRONMENTAL MICROBIOLOGY, 2003, 5 (09) :719-729

[10] Updated version of the Burkholderia cepacia complex experimental strain panel [J].

Coenye, T ;

Vandamme, P ;

LiPuma, JJ ;

Govan, JRW ;

Mahenthiralingam, E .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (06) :2797-2798

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