Synthetic Peptides Derived from Bovine Lactoferricin Exhibit Antimicrobial Activity against E-coli ATCC 11775, S-maltophilia ATCC 13636 and S-enteritidis ATCC 13076

被引：34

作者：

Huertas Mendez, Nataly De Jesus ^{[1
]}

Vargas Casanova, Yerly ^{[2
]}

Gomez Chimbi, Anyelith Katherine ^{[2
]}

Hernandez, Edith ^{[2
]}

Leal Castro, Aura Lucia ^{[3
]}

Melo Diaz, Javier Mauricio ^{[1
]}

Rivera Monroy, Zuly Jenny ^{[1
]}

Garcia Castaneda, Javier Eduardo ^{[4
]}

机构：

[1] Univ Nacl Colombia, Dept Chem, Bogota Carrera 45 26-85,Bldg 451,Off 409, Bogota 11321, Colombia

[2] Univ Colegio Mayor Cundinamarca, Dept Bacteriol, Bogota Calle 28 5B-02, Bogota 110311, Colombia

[3] Univ Nacl Colombia, Fac Med, Bogota Carrera 45 26-85,Bldg 471, Bogota 11321, Colombia

[4] Univ Nacl Colombia, Dept Pharm, Bogota Carrera 45 26-85,Bldg 450,Off 203, Bogota 11321, Colombia

来源：

MOLECULES | 2017年 / 22卷 / 03期

关键词：

Lactoferricin B; E; coli; S; maltophilia; enteritidis; antibacterial activity; synthetic peptides; ANTIBACTERIAL ACTIVITY; STENOTROPHOMONAS-MALTOPHILIA; CYTOPLASMIC MEMBRANE; SUSCEPTIBILITY; DERIVATIVES; BACTERIAL; INHIBIT; PROTEIN; RICH;

D O I：

10.3390/molecules22030452

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Linear, dimeric, tetrameric, and cyclic peptides derived from lactoferricin B-containing non-natural amino acids and the RWQWR motif were synthesized, purified, and characterized using RP-HPLC, MALDI-TOF mass spectrometry, and circular dichroism. The antibacterial activity of peptides against Escherichia coli ATCC 11775, Stenotrophomonas maltophilia ATCC 13636, and Salmonella enteritidis ATCC 13076 was evaluated. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. The synthetic bovine lactoferricin exhibited antibacterial activity against E. coli ATCC 11775 and S. enteritidis ATCC 13076. The dimeric peptide (RRWQWR)(2)K-Ahx exhibited the highest antibacterial activity against the tested bacterial strain. The monomeric, cyclic, tetrameric, and palindromic peptides containing the RWQWR motif exhibited high and specific activity against E. coli ATCC 11775. The results suggest that short peptides derived from lactoferricin B could be considered as potential candidates for the development of antibacterial agents against infections caused by E. coli.

引用

页数：10

共 54 条

[21] Bulky aromatic amino acids increase the antibacterial activity of 15-residue bovine lactoferricin derivatives [J].