Physiological and biochemical characteristics of tobacco transgenic plants expressing bacterial dioxygenase

被引:4
作者
Piruzian, ES
Goldenkova, IV
Lenets, AA
Cvikrová, M
Machácková, I
Kobets, NS
Mett, VL
Musiichuk, KA
机构
[1] Russian Acad Sci, NI Vavilov Gen Genet Res Inst, Moscow 119991, Russia
[2] Natl Acad Sci, Bot Gardens, Minsk, BELARUS
[3] Acad Sci Czech Republ, Inst Expt Bot, Prague, Czech Republic
关键词
transgenic plants; phenolic compounds; 1,2-dihydroxynaphthalene dioxygenase polyphenol oxidases;
D O I
10.1023/A:1020977931969
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Expression of the 1,2-dihydroxynaphthalene dioxygenase (nahC) gene from Pseudomonas putida in tobacco transgenic plants produces notable phenotypic and biochemical changes: retarded growth and rooting and earlier flowering; chlorotic and necrotic spots on leaves; and a threefold increase in the total phenolics in the leaves of 6-week-old plants (94.51 mug/g fr wt as compared to 33.18 mug/g fr wt in the control) and in the phenylalanine-ammonia lyase activity in 4-week-old plants (0.035 U/g fr wt as compared to 0.014 U/g in the control plants of the same age). The transgenic plants expressing the nahC bacterial gene may serve as a model to study the putative functions of dioxygenases and phenol compounds in plant growth, development, and stress responses.
引用
收藏
页码:817 / 822
页数:6
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