Evaluation of Methanol Induced Free Radicals in Mice Liver

被引：1
作者：
Zhou, Lifang [1 ,2 ]
Zhao, Hongli [1 ]
Pan, Tieying [2 ]
Trinchi, Adrian [3 ]
Lan, Minbo [1 ,2 ]
Wei, Gang [1 ,4 ]
机构：
[1] East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China
[2] East China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China
[3] CSIRO Mfg, Private Bag 10, Clayton, Vic 3169, Australia
[4] CSIRO Mfg, POB 218, Lindfield, NSW 2070, Australia
来源：
AUSTRALIAN JOURNAL OF CHEMISTRY | 2017年 / 70卷 / 05期
关键词：
ELECTRON-PARAMAGNETIC-RESONANCE; OXIDATIVE STRESS; IN-VIVO; CARASSIUS-AURATUS; HYDROXYL RADICALS; RAT; EPR; GENERATION; TOXICITY; MODEL;
D O I：
10.1071/CH16492
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Methanol induced oxidative stress (OS) models in mice were successfully established and evaluated by the electron paramagnetic resonance (EPR) spin trapping technique. The capacity for removal of reactive oxygen species (ROS) free radicals by rhubarb and vitamin C (Vc) as candidate materials was also investigated. EPR was employed to determine the free radicals generated from a spin trapping agent, alpha-phenyl-N-tert-butylnitrone (PBN), that reacted with the ROS. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), and the level of malondialdehyde (MDA) were also evaluated by enzyme assays. The results indicated that methanol clearly promoted the generation of ROS free radicals in the liver of mice. The activity of SOD and GSH-PX was reduced significantly, although the level of MDA was increased as a result of the harmful effect of methanol. In addition, rhubarb and Vc exhibited a protective effect on the mice liver under acute OS.
引用
收藏
页码：499 / 504
页数：6
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