GDP-d-mannose 3,5-epimerase (GME) plays a key role at the intersection of ascorbate and non-cellulosic cell-wall biosynthesis in tomato

被引:169
作者
Gilbert, Louise [1 ]
Alhagdow, Moftah [1 ]
Nunes-Nesi, Adriano [2 ]
Quemener, Bernard [3 ]
Guillon, Fabienne [3 ]
Bouchet, Brigitte [3 ]
Faurobert, Mireille [4 ]
Gouble, Barbara [5 ]
Page, David [5 ]
Garcia, Virginie [1 ]
Petit, Johann [1 ]
Stevens, Rebecca [4 ]
Causse, Mathilde [4 ]
Fernie, Alisdair R. [2 ]
Lahaye, Marc [3 ]
Rothan, Christophe [1 ]
Baldet, Pierre [1 ]
机构
[1] Univ Bordeaux, INRA, Inst Fed Rech 103, UMR Biol Fruit 619, BP 81, F-33883 Villenave Dornon, France
[2] Max Planck Inst Mol Pflanzenphysiol, D-14467 Potsdam, Germany
[3] INRA, Unite Biopolymeres Interact Assemblage, UR1268, F-44316 Nantes, France
[4] INRA, Unite Genet & Ameliorat Fruits & Legumes, UR1052, F-84143 Montfavet, France
[5] INRA, Unite Securite & Qualite Prod Origine Vegetale, UMR408, F-84914 Avignon, France
关键词
GDP-d-mannose; 3; 5-epimerase; ascorbate; cell wall; tomato fruit; Solanum lycopersicum; STRUCTURALLY ALTERED GALACTOMANNANS; VITAMIN-C; RHAMNOGALACTURONAN-II; NOVO BIOSYNTHESIS; PLANTS; FRUIT; POLYSACCHARIDES; MUTANT; LOCALIZATION; REQUIREMENT;
D O I
10.1111/j.1365-313X.2009.03972.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
P>The GDP-d-mannose 3,5-epimerase (GME, EC 5.1.3.18), which converts GDP-d-mannose to GDP-l-galactose, is generally considered to be a central enzyme of the major ascorbate biosynthesis pathway in higher plants, but experimental evidence for its role in planta is lacking. Using transgenic tomato lines that were RNAi-silenced for GME, we confirmed that GME does indeed play a key role in the regulation of ascorbate biosynthesis in plants. In addition, the transgenic tomato lines exhibited growth defects affecting both cell division and cell expansion. A further remarkable feature of the transgenic plants was their fragility and loss of fruit firmness. Analysis of the cell-wall composition of leaves and developing fruit revealed that the cell-wall monosaccharide content was altered in the transgenic lines, especially those directly linked to GME activity, such as mannose and galactose. In agreement with this, immunocytochemical analyses showed an increase of mannan labelling in stem and fruit walls and of rhamnogalacturonan labelling in the stem alone. The results of MALDI-TOF fingerprinting of mannanase cleavage products of the cell wall suggested synthesis of specific mannan structures with modified degrees of substitution by acetate in the transgenic lines. When considered together, these findings indicate an intimate linkage between ascorbate and non-cellulosic cell-wall polysaccharide biosynthesis in plants, a fact that helps to explain the common factors in seemingly unrelated traits such as fruit firmness and ascorbate content.
引用
收藏
页码:499 / 508
页数:10
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