Functional crosstalk between phospholipase D2 and signaling phospholipase A2/cyclooxygenase-2-medisted prostaglandin biosynthetic pathways

We performed reconstitution analyses of functional interaction between phospholipase A(2) (PLA(2)) and phospholipase D (PLD) enzymes. Cotransfection of HEK293 cells with cytosolic (cPLA(2)) or type IIA secretory (sPLA(2)-IIA) PLA(2) and PLD2, but not PLD1, led to marked augmentation of stimulus-induced arachidonate release, Interleukin-1-stimulated arachidonate release was accompanied by prostaglandin E-2 production via cyclooxygenase-2, the expression of which was augmented by PLD2. Conversely, activation of PLD2, not PLD1, was facilitated by cPLA(2) or sPLA(2)-IIA, Thus, our results revealed functional crosstalk between signaling PLA(2)s and PLD2 in the regulation of various cellular responses in which these enzymes have been implicated. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.