Purification and characterization of soluble peroxidase from oil palm (Elaeis guineensis Jacq.) leaf

被引：58
|
作者：
Deepa, SS [1 ]
Arumughan, C [1 ]
机构：
[1] CSIR, Reg Res Lab, Agro Proc Div, Trivandrum 695019, Kerala, India
来源：
PHYTOCHEMISTRY | 2002年 / 61卷 / 05期
关键词：
Elaeis guineensis Jacq; oil palm; leaflet; enzyme purification; peroxidase; vascular bundle; xylem; phloem;
D O I：
10.1016/S0031-9422(02)00167-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Soluble peroxidase (POD) from oil palm leaf was purified by (NH4)(2)SO4 precipitation, anion exchange chromatography and molecular exclusion chromatography. The purification grade obtained was 429 yielding 54% of the enzyme activity. Electrophoresis of purified enzyme under denatured conditions revealed M-r of 48 +/- 2 kDa. It has an optimum pH of 5 and it exhibited very high pH and thermal stabilities. K-m for guaiacol, ABTS and pyrogallol were 3.96, 1 and 0.84 mM, respectively. Immunocytochemical localization studies showed that soluble POD was mainly located in the vascular bundles and epidermis of leaf. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：503 / 511
页数：9
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