A High-Affinity Fluorescent Sensor for Catecholamine: Application to Monitoring Norepinephrine Exocytosis

被引：99
作者：
Zhang, Le [1 ]
Liu, Xin A. [2 ,3 ]
Gillis, Kevin D. [2 ,3 ]
Glass, Timothy E. [1 ]
机构：
[1] Univ Missouri, Dept Chem, Columbia, MO 65211 USA
[2] Univ Missouri, Dalton Cardiovasc Res Ctr, Dept Bioengn, Columbia, MO 65211 USA
[3] Univ Missouri, Dept Med Pharmacol & Physiol, Columbia, MO 65211 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2019年 / 58卷 / 23期
基金：
美国国家卫生研究院;
关键词：
amperometry; catecholamine; fluorescent Sensor; neuroscience; DOPAMINE RELEASE; NEUROTRANSMITTERS; RECOGNITION; DESIGN; CELLS;
D O I：
10.1002/anie.201810919
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A fluorescent sensor for catecholamines, NS510, is presented. The sensor is based on a quinolone fluorophore incorporating a boronic acid recognition element that gives it high affinity for catecholamines and a turn-on response to norepinephrine. The sensor results in punctate staining of norepinephrine-enriched chromaffin cells visualized using confocal microscopy indicating that it stains the norepinephrine in secretory vesicles. Amperometry in conjunction with total internal reflection fluorescence (TIRF) microscopy demonstrates that the sensor can be used to observe destaining of individual chromaffin granules upon exocytosis. NS510 is the highest affinity fluorescent norepinephrine sensor currently available and can be used for measuring catecholamines in live-cell assays.
引用
收藏
页码：7611 / 7614
页数：4
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