A bioactive injectable self-healing anti-inflammatory hydrogel with ultralong extracellular vesicles release synergistically enhances motor functional recovery of spinal cord injury

被引：99

作者：

Wang, Chenggui ^{[1
]}

Wang, Min ^{[2
]}

Xia, Kaishun ^{[1
]}

Wang, Jingkai ^{[1
]}

Cheng, Feng ^{[1
]}

Shi, Kesi ^{[1
]}

Ying, Liwei ^{[1
]}

Yu, Chao ^{[1
]}

Xu, Haibin ^{[1
]}

Xiao, Shining ^{[1
]}

Liang, Chengzhen ^{[1
]}

Li, Fangcai ^{[1
]}

Lei, Bo ^{[2
]}

Chen, Qixin ^{[1
]}

机构：

[1] Zhejiang Univ, Affiliated Hosp 2, Sch Med, Dept Orthoped Surg, Hangzhou 310009, Zhejiang, Peoples R China

[2] Xi An Jiao Tong Univ, Coll Stomatol, Frontier Inst Sci & Technol, Key Lab Shaanxi Prov Craniofacial Precis Med Res, Xian 710054, Peoples R China

来源：

BIOACTIVE MATERIALS | 2021年 / 6卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Bioactive biomaterials; Multifunctional hydrogel; Extracellular vesicles release; Spinal cord injury repair; QUALITY-OF-LIFE; MACROPHAGE DEPLETION; FIBROTIC SCAR; DIFFERENTIATION; REGENERATION; DELIVERY; INFLAMMATION; INDIVIDUALS; EXPRESSION; APOPTOSIS;

D O I：

10.1016/j.bioactmat.2021.01.029

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The repair and motor functional recovery after spinal cord injury (SCI) remains a worldwide challenge. The inflammatory microenvironment is one of main obstacles on inhibiting the recovery of SCI. Using mesenchymal stem cells (MSCs) derived extracellular vesicles to replace MSCs transplantation and mimic cell paracrine secretions provides a potential strategy for microenvironment regulation. However, the effective preservation and controlled release of extracellular vesicles in the injured spinal cord tissue are still not satisfied. Herein, we fabricated an injectable adhesive anti-inflammatory F127-polycitrate-polyethyleneimine hydrogel (FE) with sustainable and long term extracellular vesicle release (FE@EVs) for improving motor functional recovery after SCI. The orthotopic injection of FE@EVs hydrogel could encapsulate extracellular vesicles on the injured spinal cord, thereby synergistically induce efficient integrated regulation through suppressing fibrotic scar formation, reducing inflammatory reaction, promoting remyelination and axonal regeneration. This study showed that combining extracellular vesicles into bioactive multifunctional hydrogel should have great potential in achieving satisfactory locomotor recovery of central nervous system diseases.

引用

页码：2523 / 2534

页数：12

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