Age-Based Variations in the Gut Microbiome of the Shennongjia (Hubei) Golden Snub-Nosed Monkey (Rhinopithecus roxellana hubeiensis)

被引:10
作者
Yao, Lijuan [1 ]
Li, Xiang [1 ,2 ,3 ]
Zhou, Zutao [1 ]
Shi, Deshi [1 ]
Li, Zili [1 ]
Li, Shangfei [1 ]
Yao, Hui [4 ]
Yang, Jingyuan [4 ]
Yu, Huiliang [4 ]
Xiao, Yuncai [1 ]
机构
[1] Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China
[2] Huazhong Agr Univ, Natl Ctr Int Res Anim Genet Breeding & Reprod, Wuhan 430070, Peoples R China
[3] Huazhong Agr Univ, Coll Anim Sci & Technol, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China
[4] Hubei Conservat & Res Ctr Golden Monkey, Hubei Prov Key Lab Conservat Biol Shennongjia Gol, Shennongjia 442411, Hubei, Peoples R China
关键词
RNA GENE DATABASE; INTESTINAL MICROBIOTA; PHYLOGENETIC ANALYSIS; NATURE-RESERVE; DIVERSITY; BACTERIAL; DIET; SUPPLEMENTATION; LIGNOCELLULOSE; STRYKERI;
D O I
10.1155/2021/6667715
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The gut microbiota represents a source of genetic and metabolic diversity of a complex polymicrobial ecosystem within its host. To investigate age-based variations of the gut microbiota among Shennongjia golden snub-nosed monkeys (Rhinopithecus roxellana hubeiensis), we characterized the microbial species in fecal samples from 18 Shennongjia golden snub-nosed monkeys evenly pooled into 3 aged groups (Group 1, 1-3 years; Group 2, 5-8 years; Group 3, above 12 years) in Shennongjia, Hubei Province, China. Genomic DNA was extracted from fecal samples, and the 16S rRNA gene V4 region was sequenced using the Illumina high-throughput MiSeq platform PE250. A total of 28 microbial phyla were identified in the gut microbiome of these monkeys with the ten most abundant phyla (i.e., Firmicutes, Bacteroidetes, Verrucomicrobia, Spirochaetes, Tenericutes, Proteobacteria, Planctomycetes, Fibrobacteres, Cyanobacteria, and Euryarchaeota). A total of 1,469 (of 16 phyla and 166 genera), 1,381 (of 16 phyla and 157 genera), and 1,931 (of 19 phyla and 190 genera) operational taxonomic units (OTUs) were revealed in Groups 1, 2, and 3, respectively, with Group 3 containing the most diverse groups of OTUs as revealed by the species relative abundance clustering analysis. These results suggest that the gut microbiota in these monkeys maintain a dynamic status, starting from the early developmental stages of life with the species relative abundance increasing with age. This is the first study to comprehensively characterize the gut microbiota and provide valuable information for monitoring the health and nutritional needs of this endangered primate at different ages.
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页数:17
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共 61 条
  • [2] Oral supplementation with Lactobacillus delbrueckii subsp bulgaricus 8481 enhances systemic immunity in elderly subjects
    Antonio Moro-Garcia, Marco
    Alonso-Arias, Rebeca
    Baltadjieva, Maria
    Fernandez Benitez, Carlos
    Fernandez Barrial, Manuel Amadeo
    Diaz Ruisanchez, Enrique
    Alonso Santos, Ricardo
    Alvarez Sanchez, Magdalena
    Saavedra Mijan, Juan
    Lopez-Larrea, Carlos
    [J]. AGE, 2013, 35 (04) : 1311 - 1326
  • [3] Host-bacterial mutualism in the human intestine
    Bäckhed, F
    Ley, RE
    Sonnenburg, JL
    Peterson, DA
    Gordon, JI
    [J]. SCIENCE, 2005, 307 (5717) : 1915 - 1920
  • [4] Microbes inside-from diversity to function: the case of Akkermansia
    Belzer, Clara
    de Vos, Willem M.
    [J]. ISME JOURNAL, 2012, 6 (08) : 1449 - 1458
  • [5] Through Ageing, and Beyond: Gut Microbiota and Inflammatory Status in Seniors and Centenarians
    Biagi, Elena
    Nylund, Lotta
    Candela, Marco
    Ostan, Rita
    Bucci, Laura
    Pini, Elisa
    Nikkila, Janne
    Monti, Daniela
    Satokari, Reetta
    Franceschi, Claudio
    Brigidi, Patrizia
    De Vos, Willem
    [J]. PLOS ONE, 2010, 5 (05):
  • [6] The intestinal microbiota: its role in health and disease
    Biedermann, Luc
    Rogler, Gerhard
    [J]. EUROPEAN JOURNAL OF PEDIATRICS, 2015, 174 (02) : 151 - 167
  • [7] High Oscillospira abundance indicates constipation and low BMI in the Guangdong Gut Microbiome Project
    Chen, Yi-ran
    Zheng, Hui-min
    Zhang, Guo-xia
    Chen, Fang-lan
    Chen, Li-dan
    Yang, Zhi-cong
    [J]. SCIENTIFIC REPORTS, 2020, 10 (01)
  • [8] Underprescription of beneficial medicines in older people: Causes, consequences and prevention
    Cherubini A.
    Corsonello A.
    Lattanzio F.
    [J]. Drugs & Aging, 2012, 29 (6) : 463 - 475
  • [9] The interplay between the intestinal microbiota and the brain
    Collins, Stephen M.
    Surette, Michael
    Bercik, Premysl
    [J]. NATURE REVIEWS MICROBIOLOGY, 2012, 10 (11) : 735 - 742
  • [10] Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB
    DeSantis, T. Z.
    Hugenholtz, P.
    Larsen, N.
    Rojas, M.
    Brodie, E. L.
    Keller, K.
    Huber, T.
    Dalevi, D.
    Hu, P.
    Andersen, G. L.
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (07) : 5069 - 5072