Effect of caffeine, cAMP and cattle seminal plasma on freezability of buffalo bull semen

被引:7
|
作者
Singh, P [1 ]
Raina, VS [1 ]
机构
[1] Natl Dairy Res Inst, Artificial Inseminat Lab, Karnal 132001, Haryana, India
来源
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES | 2000年 / 13卷 / 07期
关键词
buffalo semen; sperm motility; non-eosinophilic; caffeine; cAMP; cattle seminal plasma; freezability;
D O I
10.5713/ajas.2000.901
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
An experiment was conducted to investigate the effect of caffeine, cAMP and cattle seminal plasma on preservation of semen at ultra low temperature (-196 degrees C). Each semen sample was divided into four parts equal in volume and sperm concentration; three were treated with caffeine, or cAMP, or cattle seminal plasma (CSP) and the fourth was kept as control. Sperm motility, abnormal spermatozoa, live-dead count and acrosomal damage were studied at different stages of freeze preservation viz.; just after dilution, at 5 degrees C, at glycerolisation, before freezing, just after freezing, 24 hours of storage, and one week of storage. Sperm motility (58.39, 61.33, 52.00 and 50.39 per cent), non-eosinophilic spermatozoa (72.55, 69.98, 63.31 and 67.64 per cent), abnormal spermatozoa (5.71, 4.98, 8.04 and 5.66 per cent) and acrosomal damage (13.28, 13.33, 14.80 and 14.65 per cent) were observed in cAMP, caffeine, cattle seminal plasma and control, respectively, at every stage of freeze preservation. From this study it could be concluded that freezability of buffalo semen can be improved through the addition of caffeine followed by cAMP and cattle seminal plasma.
引用
收藏
页码:901 / 905
页数:5
相关论文
共 50 条
  • [41] EFFECT OF SPERMS FREEZABILITY ON SOME SEMEN ATTRIBUTES AND AMINO ACID CONCENTRATIONS IN SEMINAL PLASMA OF HOLSTEIN BULLS
    Abdulkareem, T. A.
    Khalil, R. I.
    Al-Ezzi, M. A. O.
    IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 2024, 55 (02): : 665 - 674
  • [42] Association of oxidative status and semen characteristics with seminal plasma proteins of buffalo semen
    Sharma, L.
    Pandey, V
    Nigam, R.
    Saxena, A.
    Swain, D. K.
    Yadav, B.
    IRANIAN JOURNAL OF VETERINARY RESEARCH, 2016, 17 (04) : 226 - 230
  • [43] INFLUENCE OF SEMEN COLLECTION TECHNIQUES ON COMPOSITION OF BULL SEMINAL PLASMA
    SEIDEL, GE
    FOOTE, RH
    JOURNAL OF DAIRY SCIENCE, 1969, 52 (07) : 1080 - &
  • [44] Effect of environmental temperature on semen quality and seminal plasma metabolites of Mediterranean buffalo bulls
    Sun, Le
    Cui, Zhichao
    Huang, Shihai
    Xue, Qingsong
    Rehman, Saif Ur
    Luo, Xi
    Shi, Deshun
    Li, Xiangping
    ANIMAL BIOTECHNOLOGY, 2022, 33 (05) : 970 - 980
  • [45] Effect of egg yolk and seminal plasma heparin binding protein interaction on the freezability of buffalo cauda epididymal spermatozoa
    Singh, L. P.
    Harshan, Hiron M.
    Ansari, M. R.
    ANIMAL REPRODUCTION SCIENCE, 2007, 99 (3-4) : 395 - 400
  • [46] Effect of peak winter and summer season on quality and freezability of Tharparkar bull semen
    Rajoriya, J. S.
    Prasad, J. K.
    Ghosh, S. K.
    Kumar, Anuj
    Ramteke, Snehal S.
    Singh, Mahak
    Kumar, Sandeep
    INDIAN JOURNAL OF ANIMAL RESEARCH, 2015, 49 (02) : 265 - 268
  • [47] Freezability of water buffalo bull (Bubalus bubalis) spermatozoa is improved with the addition of curcumin (diferuoyl methane) in semen extender
    Shah, S. A. H.
    Andrabi, S. M. H.
    Qureshi, I. Z.
    ANDROLOGIA, 2017, 49 (08)
  • [48] Effects of removal of seminal plasma on the survival rate of buffalo bull spermatozoa
    Ahmad, M
    Khan, A
    Shah, ZA
    Ahmad, KM
    ANIMAL REPRODUCTION SCIENCE, 1996, 41 (3-4) : 193 - 199
  • [49] Comparative proteomic analysis of seminal plasma proteins in relation to freezability of Dezhou donkey semen
    Yu, Jie
    Li, Min
    Ji, Chuanliang
    Li, Xuexian
    Li, Haijing
    Liu, Guiqin
    Wang, Yantao
    Liu, Guangyuan
    Wang, Tao
    Che, Xiaonan
    Lei, Chuzhao
    Dang, Ruihua
    Zhao, Fuwei
    ANIMAL REPRODUCTION SCIENCE, 2021, 231
  • [50] Effect of multiple showering and artificial aeration on sexual behaviour, quality and freezability of buffalo bull semen during hot-humid season
    Singh, P
    Singh, N
    INDIAN JOURNAL OF ANIMAL SCIENCES, 2000, 70 (09): : 914 - 916