Effect of caffeine, cAMP and cattle seminal plasma on freezability of buffalo bull semen

被引:7
|
作者
Singh, P [1 ]
Raina, VS [1 ]
机构
[1] Natl Dairy Res Inst, Artificial Inseminat Lab, Karnal 132001, Haryana, India
来源
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES | 2000年 / 13卷 / 07期
关键词
buffalo semen; sperm motility; non-eosinophilic; caffeine; cAMP; cattle seminal plasma; freezability;
D O I
10.5713/ajas.2000.901
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
An experiment was conducted to investigate the effect of caffeine, cAMP and cattle seminal plasma on preservation of semen at ultra low temperature (-196 degrees C). Each semen sample was divided into four parts equal in volume and sperm concentration; three were treated with caffeine, or cAMP, or cattle seminal plasma (CSP) and the fourth was kept as control. Sperm motility, abnormal spermatozoa, live-dead count and acrosomal damage were studied at different stages of freeze preservation viz.; just after dilution, at 5 degrees C, at glycerolisation, before freezing, just after freezing, 24 hours of storage, and one week of storage. Sperm motility (58.39, 61.33, 52.00 and 50.39 per cent), non-eosinophilic spermatozoa (72.55, 69.98, 63.31 and 67.64 per cent), abnormal spermatozoa (5.71, 4.98, 8.04 and 5.66 per cent) and acrosomal damage (13.28, 13.33, 14.80 and 14.65 per cent) were observed in cAMP, caffeine, cattle seminal plasma and control, respectively, at every stage of freeze preservation. From this study it could be concluded that freezability of buffalo semen can be improved through the addition of caffeine followed by cAMP and cattle seminal plasma.
引用
收藏
页码:901 / 905
页数:5
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