Multiplex real-time PCR using SYBR® GreenER™ for the detection of DNA allergens in food

被引：66

作者：

Pafundo, Simona ^{[1
]}

Gulli, Mariolina ^{[1
]}

Marmiroli, Nelson ^{[1
]}

机构：

[1] Univ Parma, Dept Environm Sci, Div Genet & Environm Biotechnol, I-43100 Parma, Italy

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2010年 / 396卷 / 05期

关键词：

Allergen; Multiplex real-time PCR; Traceability; Food; Melting curve; POLYMERASE-CHAIN-REACTION; MELTING CURVE ANALYSIS; GREEN-I; HAZELNUT; SESAME; VIRUS; ASSAY; DIFFERENTIATION; HYBRIDIZATION; PATHOGENS;

D O I：

10.1007/s00216-009-3419-z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the development of a six-target realtime multiplex PCR assay with the SYBR(R) GreenER(TM) fluorescent dye, targeted to genes encoding for allergenic proteins commonly present in many processed food products (patent application pending). The assay was successfully trialled on reconstructed food matrices and on a range of commercial foodstuffs, and is proposed as a ready-to-use analytical tool for food manufacturers to detect the presence or confirm the absence of sequences encoding for important allergenic proteins of plant origin.

引用

页码：1831 / 1839

页数：9

共 38 条

[21] Novel multiplex PCR for the detection of the Staphylococcus aureus superantigen and its application to raw meat isolates in Korea [J].

Hwang, Sun Young ;

Kim, So Hyun ;

Jang, Eun Joo ;

Kwon, Nam Hoon ;

Park, Young Kyung ;

Koo, Hye Cheong ;

Jung, Woo Kyung ;

Kim, Jun Man ;

Park, Yong Ho .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2007, 117 (01) :99-105

[22] Molecular diagnosis of α-thalassemia by combining real-time PCR with SYBR Green1 and dissociation curve analysis [J].

Liu, Jingzhong ;

Yan, Mei ;

Wang, Zhangyong ;

Wang, Lirong ;

Zhou, Yan ;

Xiao, Bai .

TRANSLATIONAL RESEARCH, 2006, 148 (01) :6-12

[23] Polymerase chain reaction (PCR) in the quality and safety assurance of food: Detection of soya in processed meat products [J].

Meyer, R ;

Chardonnens, F ;

Hubner, P ;

Luthy, J .

ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG, 1996, 203 (04) :339-344

[24] Development of a multiplex real-time PCR assay using SYBR Green 1 chemistry for simultaneous detection and subtyping of H9N2 influenza virus type A [J].

Ong, Wee Theng ;

Omar, Abdul Rahman ;

Ideris, Aini ;

Hassan, Sharifah Syed .

JOURNAL OF VIROLOGICAL METHODS, 2007, 144 (1-2) :57-64

[25] SYBR®GreenER™ Real-Time PCR to detect almond in traces in processed food [J].

Pafundo, Simona ;

Gulli, Mariolina ;

Marmiroli, Nelson .

FOOD CHEMISTRY, 2009, 116 (03) :811-815

[26] Multiplex polymerase chain reaction and ligation detection reaction/universal array technology for the traceability of genetically modified organisms in foods [J].

Peano, C ;

Bordoni, R ;

Gulli, M ;

Mezzelani, A ;

Samson, MC ;

De Bellis, G ;

Marmiroli, N .

ANALYTICAL BIOCHEMISTRY, 2005, 346 (01) :90-100

[27] Rapid detection and differentiation of Newcastle disease virus by real-time PCR with melting-curve analysis [J].

Pham, HM ;

Konnai, S ;

Usui, T ;

Chang, KS ;

Murata, S ;

Mase, M ;

Ohashi, K ;

Onuma, M .

ARCHIVES OF VIROLOGY, 2005, 150 (12) :2429-2438

[28] Methods for allergen analysis in food: a review [J].

Poms, RE ;

Klein, CL ;

Anklam, E .

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2004, 21 (01) :1-31

[29] LightCycler multiplex PCR for the laboratory diagnosis of common viral infections of the central nervous system [J].

Read, SJ ;

Mitchell, JL ;

Fink, CG .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (09) :3056-3059

[30] Development and Validation of a Duplex Real-Time PCR Method To Simultaneously Detect Potentially Allergenic Sesame and Hazelnut in Food [J].

Schoeringhumer, Kerstin ;

Redl, Gerda ;

Cichna-Markl, Margit .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2009, 57 (06) :2126-2134

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