Multiplex real-time PCR using SYBR® GreenER™ for the detection of DNA allergens in food

被引：66

作者：

Pafundo, Simona ^{[1
]}

Gulli, Mariolina ^{[1
]}

Marmiroli, Nelson ^{[1
]}

机构：

[1] Univ Parma, Dept Environm Sci, Div Genet & Environm Biotechnol, I-43100 Parma, Italy

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2010年 / 396卷 / 05期

关键词：

Allergen; Multiplex real-time PCR; Traceability; Food; Melting curve; POLYMERASE-CHAIN-REACTION; MELTING CURVE ANALYSIS; GREEN-I; HAZELNUT; SESAME; VIRUS; ASSAY; DIFFERENTIATION; HYBRIDIZATION; PATHOGENS;

D O I：

10.1007/s00216-009-3419-z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the development of a six-target realtime multiplex PCR assay with the SYBR(R) GreenER(TM) fluorescent dye, targeted to genes encoding for allergenic proteins commonly present in many processed food products (patent application pending). The assay was successfully trialled on reconstructed food matrices and on a range of commercial foodstuffs, and is proposed as a ready-to-use analytical tool for food manufacturers to detect the presence or confirm the absence of sequences encoding for important allergenic proteins of plant origin.

引用

页码：1831 / 1839

页数：9

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