Synergistic bactericidal effect of hot water with citric acid against Escherichia coli O157:H7 biofilm formed on stainless steel

被引:38
作者
Kang, Jun-Won [1 ,2 ]
Lee, Hae-Yeon [1 ,2 ]
Kang, Dong-Hyun [1 ,2 ]
机构
[1] Seoul Natl Univ, Res Inst Agr & Life Sci, Dept Food & Anim Biotechnol, Dept Agr Biotechnol,Ctr Food & Bioconvergence, Seoul 08826, South Korea
[2] Seoul Natl Univ, Inst Green Bio Sci & Technol, Gangwon Do 25354, South Korea
基金
新加坡国家研究基金会;
关键词
Biofilm; Escherichia coli O157:H7; Hot water; Citric acid; Synergistic effect; Stainless steel; LISTERIA-MONOCYTOGENES BIOFILMS; ENTERICA-SEROVAR TYPHIMURIUM; FOOD-CONTACT SURFACES; LIPID-PEROXIDATION; SUBLETHAL INJURY; LACTIC-ACID; SALMONELLA; INACTIVATION; RESISTANCE; O157-H7;
D O I
10.1016/j.fm.2020.103676
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This study investigated the antimicrobial effect of hot water with citric acid against Escherichia coli O157:H7 biofilm on stainless steel (SS). Hot water (50, 60, or 70 degrees C) with 2% citric acid exhibited a synergistic bactericidal effect on the pathogen biofilm. It was revealed that hot water and citric acid combination induced sub-lethally injured cells. Additionally, mechanisms of the synergistic bactericidal effects of hot water with citric acid were identified through several approaches. In terms of biofilm matrix, hot water removes exopolysaccharides, a major component of extracellular polymeric substances (EPS), thereby increasing contact between surface cells and citric acid, resulting in a synergistic bactericidal effect. In terms of the cell itself, increased permeability of citric acid through cell membranes destructed by hot water promotes the inactivation of superoxide dismutase (SOD) in E. coli O157:H7, which induce synergistic generation of reactive oxygen species (ROS) which promote inactivation of cell by activating lipid peroxidation, resulting in destruction of the cell membrane. Therefore, it is interpreted that when hot water with citric acid is applied to E. coli O157:H7 biofilm, synergy effects on the biofilm matrix and cell itself have a complex interaction with each other, thus causing a dramatic synergistic bactericidal effect.
引用
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页数:8
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