Molecular DNA identification of medicinal plants used by traditional healers in Malaysia

被引：11

作者：

Aziz, N. A. A. ^{[1
]}

Ahmad, M. I. ^{[1
]}

Naim, D. M. ^{[2
]}

机构：

[1] Univ Sains Malaysia, Sch Ind Technol, George Town, Malaysia

[2] Univ Sains Malaysia, Sch Biol Sci, George Town, Malaysia

来源：

GENETICS AND MOLECULAR RESEARCH | 2015年 / 14卷 / 04期

关键词：

DNA barcode; Medicinal plant; ITS2; rpoC1; trnH-psbA; BARCODES; REVEALS;

D O I：

10.4238/2015.December.7.5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plants have been used throughout human history for food and medicine. However, many plants are toxic, and cannot easily be morphologically distinguished from non-toxic plants. DNA identification solves this problem and is widely used. Nonetheless, plant DNA barcode identification faces a number of challenges, and many studies have been conducted to find suitable barcodes. The present study was conducted to test the efficiency of commonly used primers, namely ITS2, rpoC1, and trnH-psbA, in order to find the best DNA barcode markers for the identification of medicinal plants in Malaysia. Fresh leaves from 12 medicinal plants that are commonly used by Malay traditional healers were collected from the Tropical Spice Garden, Pulau Pinang, and subjected to polymerase chain reaction amplification using ITS2, rpoC1, and trnH-psbA DNA markers. We found that trnH-psbA is the best DNA marker for the species-level identification of medicinal plants in Malaysia.

引用

页码：15937 / 15947

页数：11

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