Betulinic Acid Induces Apoptosis in Differentiated PC12 Cells Via ROS-Mediated Mitochondrial Pathway

被引：119

作者：

Wang, Xi ^{[1
]}

Lu, Xiaocheng ^{[2
]}

Zhu, Ronglan ^{[3
]}

Zhang, Kaixin ^{[1
,4
]}

Li, Shuai ^{[1
]}

Chen, Zhongjun ^{[1
,5
]}

Li, Lixin ^{[1
]}

机构：

[1] Nanjing Med Univ, Dept Neurosurg, Affiliated Hosp 1, 300 Guangzhou Rd, Nanjing 210029, Jiangsu, Peoples R China

[2] Soochow Univ, Dept Neurosurg, Affiliated Hosp 1, 188 Shizi St, Suzhou 215006, Jiangsu, Peoples R China

[3] Jiangxi Prov Peoples Hosp, Dept Neurosurg, 92 Aiguo Rd, Nanchang 330006, Jiangxi, Peoples R China

[4] Huangshan City Peoples Hosp, Dept Neurosurg, 4 Liyuan Rd, Huangshan 245000, Anhui, Peoples R China

[5] Second Peoples Hosp Huaian, Dept Neurosurg, 62 Huaihainan Rd, Huaian 223002, Jiangsu, Peoples R China

来源：

NEUROCHEMICAL RESEARCH | 2017年 / 42卷 / 04期

基金：

中国国家自然科学基金;

关键词：

BA; PC12; cells; Apoptosis; Mitochondrial; ROS; HUMAN-MELANOMA CELLS; LUNG-CANCER GROWTH; NEUROECTODERMAL TUMORS; INDUCED CYTOTOXICITY; GLIOMA-CELLS; ACTIVATION; GENERATION; DEATH; DERIVATIVES; INDUCTION;

D O I：

10.1007/s11064-016-2147-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Betulinic acid (BA), a pentacyclic triterpene of natural origin, has been demonstrated to have varied biologic activities including anti-viral, anti-inflammatory, and anti-malarial effects; it has also been found to induce apoptosis in many types of cancer. However, little is known about the effect of BA on normal cells. In this study, the effects of BA on normal neuronal cell apoptosis and the mechanisms involved were studied using differentiated PC12 cells as a model. Treatment with 50 mu M BA for 24 h apparently induced PC12 cell apoptosis. In the early stage of apoptosis, the level of intracellular reactive oxygen species (ROS) increased. Afterwards, the loss of the mitochondrial membrane potential, the release of cytochrome c and the activation of caspase-3 occurred. Treatment with antioxidants could significantly reduce BA-induced PC12 cell apoptosis. In conclusion, we report for the first time that BA induced the mitochondrial apoptotic pathway in differentiated PC12 cells through ROS.

引用

页码：1130 / 1140

页数：11

共 44 条

[21] Caspase Cascade Regulated Mitochondria Mediated Apoptosis in Monocrotophos Exposed PC12 Cells [J].