Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation

被引：79
作者：
Jinek, Martin [1 ]
Fabian, Marc R. [2 ,3 ]
Coyle, Scott M. [1 ,4 ]
Sonenberg, Nahum [2 ,3 ]
Doudna, Jennifer A. [1 ,4 ,5 ,6 ]
机构：
[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[2] McGill Univ, Dept Biochem, Montreal, PQ, Canada
[3] McGill Univ, Goodman Canc Ctr, Montreal, PQ, Canada
[4] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA
[5] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
[6] Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA USA
来源：
NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2010年 / 17卷 / 02期
基金：
加拿大健康研究院;
关键词：
POLY(A) BINDING-PROTEIN; TRANSLATIONAL REPRESSION; ARGONAUTE; DOMAIN; COMPLEXES; TNRC6C; CELLS; PAIP2;
D O I：
10.1038/nsmb.1768
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
GW182-family proteins are essential for microRNA-mediated translational repression and deadenylation in animal cells. Here we show that a conserved motif in the human GW182 paralog TNRC6C interacts with the C-terminal domain of polyadenylate binding protein 1 (PABC) and present the crystal structure of the complex. Mutations at the complex interface impair mRNA deadenylation in mammalian cell extracts, suggesting that the GW182-PABC interaction contributes to microRNA-mediated gene silencing.
引用
收藏
页码：238 / 240
页数：3
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