High-sensitivity protein solid-state NMR spectroscopy

被引：19
作者：
Mandala, Venkata S. [1 ]
Hong, Mei [1 ]
机构：
[1] MIT, Dept Chem, 170 Albany St, Cambridge, MA 02139 USA
来源：
CURRENT OPINION IN STRUCTURAL BIOLOGY | 2019年 / 58卷
关键词：
DYNAMIC-NUCLEAR-POLARIZATION; PROTON-DETECTED NMR; ANGLE-SPINNING NMR; ENHANCED MAS NMR; HIGH-RESOLUTION; PERDEUTERATED PROTEINS; RESONANCE ASSIGNMENT; STRUCTURAL-ANALYSIS; MEMBRANE PEPTIDES; LIPID-BILAYERS;
D O I：
10.1016/j.sbi.2019.03.027
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The sensitivity of solid-state nuclear magnetic resonance (SSNMR) spectroscopy for structural biology is significantly increased by H-1 detection under fast magic-angle spinning (MAS) and by dynamic nuclear polarization (DNP) from electron spins to nuclear spins. The former allows studies of the structure and dynamics of small quantities of proteins under physiological conditions, while the latter permits studies of large biomolecular complexes in lipid membranes and cells, protein intermediates, and protein conformational distributions. We highlight recent applications of these two emerging SSNMR technologies and point out areas for future development.
引用
收藏
页码：183 / 190
页数：8
相关论文
共 69 条
  • [1] SELECTIVE OBSERVATION OF THE INTERFACE OF HETEROGENEOUS POLYCARBONATE POLYSTYRENE BLENDS BY DYNAMIC NUCLEAR-POLARIZATION C-13 NMR-SPECTROSCOPY
    AFEWORKI, M
    MCKAY, RA
    SCHAEFER, J
    [J]. MACROMOLECULES, 1992, 25 (16) : 4084 - 4091
  • [2] Structure of fully protonated proteins by proton-detected magic-angle spinning NMR
    Andreas, Loren B.
    Jaudzems, Kristaps
    Stanek, Jan
    Lalli, Daniela
    Bertarello, Andrea
    Le Marchand, Tanguy
    Paepe, Diane Cala-De
    Kotelovica, Svetlana
    Akopjana, Inara
    Knott, Benno
    Wegner, Sebastian
    Engelke, Frank
    Lesage, Anne
    Emsley, Lyndon
    Tars, Kaspars
    Herrmann, Torsten
    Pintacuda, Guido
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (33) : 9187 - 9192
  • [3] Structure and Mechanism of the Influenza A M218-60 Dimer of Dimers
    Andreas, Loren B.
    Reese, Marcel
    Eddy, Matthew T.
    Gelev, Vladimir
    Ni, Qing Zhe
    Miller, Eric A.
    Emsley, Lyndon
    Pintacuda, Guido
    Chou, James J.
    Griffin, Robert G.
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2015, 137 (47) : 14877 - 14886
  • [4] High-resolution proton-detected NMR of proteins at very fast MAS
    Andreas, Loren B.
    Le Marchand, Tanguy
    Jaudzems, Kristaps
    Pintacuda, Guido
    [J]. JOURNAL OF MAGNETIC RESONANCE, 2015, 253 : 36 - 49
  • [5] 250 GHz CW gyrotron oscillator for dynamic nuclear polarization in biological solid state NMR
    Bajaj, Vikram S.
    Hornstein, Melissa K.
    Kreischer, Kenneth E.
    Sirigiri, Jagadishwar R.
    Woskov, Paul P.
    Mak-Jurkauskas, Melody L.
    Herzfeld, Judith
    Temkin, Richard J.
    Griffin, Robert G.
    [J]. JOURNAL OF MAGNETIC RESONANCE, 2007, 189 (02) : 251 - 279
  • [6] Dynamic nuclear polarization at 9T using a novel 250 GHz gyrotron microwave source
    Bajaj, VS
    Farrar, CT
    Hornstein, MK
    Mastovsky, I
    Vieregg, J
    Bryant, J
    Eléna, B
    Kreischer, KE
    Temkin, RJ
    Griffin, RG
    [J]. JOURNAL OF MAGNETIC RESONANCE, 2003, 160 (02) : 85 - 90
  • [7] Rapid Proton-Detected NMR Assignment for Proteins with Fast Magic Angle Spinning
    Barbet-Massin, Emeline
    Pell, Andrew J.
    Retel, Joren S.
    Andreas, Loren B.
    Jaudzems, Kristaps
    Franks, W. Trent
    Nieuwkoop, Andrew J.
    Hiller, Matthias
    Higman, Victoria
    Guerry, Paul
    Bertarello, Andrea
    Knight, Michael J.
    Felletti, Michele
    Le Marchand, Tanguy
    Kotelovica, Svetlana
    Akopjana, Inara
    Tars, Kaspars
    Stoppini, Monica
    Bellotti, Vittorio
    Bolognesi, Martino
    Ricagno, Stefano
    Chou, James J.
    Griffin, Robert G.
    Oschkinat, Hartmut
    Lesage, Anne
    Emsley, Lyndon
    Herrmann, Torsten
    Pintacuda, Guido
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2014, 136 (35) : 12489 - 12497
  • [8] Out-and-back 13C-13C scalar transfers in protein resonance assignment by proton-detected solid-state NMR under ultra-fast MAS
    Barbet-Massin, Emeline
    Pell, Andrew J.
    Jaudzems, Kristaps
    Franks, W. Trent
    Retel, Joren S.
    Kotelovica, Svetlana
    Akopjana, Inara
    Tars, Kaspars
    Emsley, Lyndon
    Oschkinat, Hartmut
    Lesage, Anne
    Pintacuda, Guido
    [J]. JOURNAL OF BIOMOLECULAR NMR, 2013, 56 (04) : 379 - 386
  • [9] Helical Conformation in the CA-SP1 Junction of the Immature HIV-1 Lattice Determined from Solid-State NMR of Virus-like Particles
    Bayro, Marvin J.
    Ganser-Pornillos, Barbie K.
    Zadrozny, Kaneil K.
    Yeager, Mark
    Tycko, Robert
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2016, 138 (37) : 12029 - 12032
  • [10] DYNAMIC NUCLEAR-POLARIZATION WITH A CYCLOTRON-RESONANCE MASER AT 5-T
    BECERRA, LR
    GERFEN, GJ
    TEMKIN, RJ
    SINGEL, DJ
    GRIFFIN, RG
    [J]. PHYSICAL REVIEW LETTERS, 1993, 71 (21) : 3561 - 3564
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