Protozoan-Induced Regulation of Cyclic Lipopeptide Biosynthesis Is an Effective Predation Defense Mechanism for Pseudomonas fluorescens

被引:93
|
作者
Mazzola, Mark [1 ]
de Bruijn, Irene [2 ]
Cohen, Michael F. [3 ]
Raaijmakers, Jos M. [2 ]
机构
[1] USDA ARS, Wenatchee, WA 98801 USA
[2] Wageningen Univ, Phytopathol Lab, Wageningen, Netherlands
[3] Sonoma State Univ, Dept Biol, Rohnert Pk, CA 94928 USA
基金
美国农业部;
关键词
AMEBAS ACANTHAMOEBA-CASTELLANII; BIOLOGICAL-CONTROL; BIOFILM FORMATION; PLANT-GROWTH; PUTISOLVIN-I; SUGAR-BEET; SOIL; BIOCONTROL; BACTERIA; TOMATO;
D O I
10.1128/AEM.01272-09
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Environmental bacteria are exposed to a myriad of biotic interactions that influence their function and survival. The grazing activity of protozoan predators significantly impacts the dynamics, diversification, and evolution of bacterial communities in soil ecosystems. To evade protozoan predation, bacteria employ various defense strategies. Soil-dwelling Pseudomonas fluorescens strains SS101 and SBW25 produce the cyclic lipopeptide surfactants (CLPs) massetolide and viscosin, respectively, in a quorum-sensing-independent manner. In this study, CLP production was shown to protect these bacteria from protozoan predation as, compared to CLP-deficient mutants, strains SS101 and SBW25 exhibited resistance to grazing by Naegleria americana in vitro and superior persistence in soil in the presence of this bacterial predator. In the wheat rhizosphere, CLP-producing strains had a direct deleterious impact on the survival of N. americana. In vitro assays further showed that N. americana was three times more sensitive to viscosin than to massetolide and that exposure of strain SS101 or SBW25 to this protozoan resulted in upregulation of CLP biosynthesis genes. Enhanced expression of the massABC and viscABC genes did not require physical contact between the two organisms as gene expression levels were up to threefold higher in bacterial cells harvested 1 cm from feeding protozoans than in cells collected 4 cm from feeding protozoans. These findings document a new natural function of CLPs and highlight that bacterium-protozoan interactions can result in activation of an antipredator response in prey populations.
引用
收藏
页码:6804 / 6811
页数:8
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