Effects of Protein Restriction and Subsequent Realimentation on Body Composition, Gut Microbiota and Metabolite Profiles in Weaned Piglets

被引:20
|
作者
Hou, Lei [1 ]
Wang, Li [2 ]
Qiu, Yueqin [2 ]
Xiong, YunXia [2 ]
Xiao, Hao [2 ]
Yi, Hongbo [2 ]
Wen, Xiaolu [2 ]
Lin, Zeling [2 ]
Wang, Zhikang [2 ]
Yang, Xuefen [2 ]
Jiang, Zongyong [1 ,2 ]
机构
[1] Northeast Agr Univ, Inst Anim Nutr, Harbin 150030, Peoples R China
[2] Guangdong Acad Agr Sci, Minist Agr,Guangdong Prov Key Lab Anim Breeding &, Key Lab Anim Nutr & Feed Sci South China,Inst Ani, Maoming Branch,Guangdong Lab Lingnan Modern Agr,S, Guangzhou 510640, Peoples R China
来源
ANIMALS | 2021年 / 11卷 / 03期
关键词
body composition; metabolite; microbiota; protein restriction; realimentation; piglet; FATTY-ACIDS FAHFAS; DIETARY-PROTEIN; GROWTH-PERFORMANCE; COLONIC MICROBIOTA; FECAL MICROBIOTA; LIPID-METABOLISM; HOST; BACTERIA; PIGS; MORPHOLOGY;
D O I
10.3390/ani11030686
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Simple Summary Protein restriction strategies are often used in weaned piglets to reduce the incidence of intestinal disorders that are sensitive to dietary protein supply, but may lead to a decline in production performance. Subsequent protein realimentation can alleviate the detrimental effects of reduced dietary protein on growth. However, the effects of protein realimentation on the body composition, gut microbiota and metabolite profiles of piglets are poorly understood. The present study, combining comparative slaughter methods, microbiome and metabolome analyses, demonstrated that protein restriction and subsequent realimentation lead to compensatory growth and compensatory protein deposition in piglets, and contribute to animal intestinal health by altering the gut microbiota and metabolite profiles. The objective of this study was to evaluate the effects of protein restriction and subsequent protein realimentation on the body composition, gut microbiota and metabolite profiles of piglets. Fifty weaned piglets were randomly assigned to two treatments: a normal protein (NP) group (20% crude protein (CP)) or a low protein (LP) group (16% CP) with five animals per pen and five pens per group. Treatment diets were fed for 14 d during the protein restriction phase, and then all pigs were fed the same nursery diets with a normal CP level (19% CP) during the protein realimentation phase until they reached an average target body weight (BW) of 25 +/- 0.15 kg. At day 14 and the end of the experiment, one piglet close to the average BW of each pen was slaughtered to determine body composition, microbial composition and microbial metabolites. Results showed that there was no difference (p > 0.05) in the experimental days to reach target BW between the LP and NP groups. The average daily gain (ADG) and gain:feed ratio (G:F) during the protein restriction phase as well as BW at day 14, were significantly decreased (p < 0.05) in the LP group compared with the NP group. However, there were no significant differences (p > 0.05) during the protein realimentation phase and the overall experiment. Similarly, piglets in the LP group showed a significantly decreased body protein content (p < 0.05) at day 14, but not (p > 0.05) at the end of the experiment. The relative abundance of Parabacteroides, Butyricicoccus, Olsenella, Succinivibrio and Pseudoramibacter were significantly increased (p < 0.05), while the relative abundance of Alloprevotella and Faecalicoccus were significantly decreased (p < 0.05) in the LP group at day 14. At the end of the experiment, the piglets in the LP group showed a higher (p < 0.05) colonic relative abundances of Parabacteroides, unidentified Christensenellaceae and Caproiciproducens, and a lower (p < 0.05) relative abundance of unidentified Prevotellaceae, Haemophilus, Marvinbryantia, Faecalibaculum, Neisseria and Dubosiella than those in the NP group. Metabolomics analyses indicated that tryptophan metabolism and vitamin metabolism were enriched in the LP group at day 14, and glycerophospholipid metabolism and fatty acid esters of hydroxy fatty acid metabolism were enriched at the end of the experiment. Moreover, Spearman's correlation analysis demonstrated that the microbial composition was highly correlated with changes in colonic metabolites. Collectively, these results indicated that protein restriction and subsequent realimentation lead to compensatory growth and compensatory protein deposition in piglets and contribute to animal intestinal health by altering the gut microbiota and its metabolites.
引用
收藏
页码:1 / 17
页数:17
相关论文
共 50 条
  • [1] Effects of early sub-therapeutic antibiotic administration on body tissue deposition, gut microbiota and metabolite profiles of weaned piglets
    Hou, Lei
    Cao, Shuting
    Qiu, Yueqin
    Xiong, YunXia
    Xiao, Hao
    Wen, Xiaolu
    Yang, Xuefen
    Gao, Kaiguo
    Wang, Li
    Jiang, Zongyong
    JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2022, 102 (13) : 5913 - 5924
  • [2] Effects of Protein Restriction and Succedent Realimentation on Jejunal Function and Bacterial Composition of Different Colonic Niches in Weaned Piglets
    Wang, Jue
    Zhu, Yizhi
    Tian, Shiyi
    Shi, Qing
    Yang, Huairong
    Wang, Jing
    Zhu, Weiyun
    FRONTIERS IN VETERINARY SCIENCE, 2022, 9
  • [3] Protein restriction and succedent realimentation affecting ileal morphology, ileal microbial composition and metabolites in weaned piglets
    Shi, Q.
    Zhu, Y.
    Wang, J.
    Yang, H.
    Zhu, W.
    ANIMAL, 2019, 13 (11) : 2463 - 2472
  • [4] Significant changes in caecal microbial composition and metabolites of weaned piglets after protein restriction and succedent realimentation
    Tian, Shiyi
    Shi, Qing
    Zhu, Yizhi
    Yang, Huairong
    Wang, Jing
    Zhu, Weiyun
    JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION, 2020, 104 (04) : 1126 - 1133
  • [5] Gut Microbiota-Derived Metabolite Signature in Suckling and Weaned Piglets
    Beaumont, Martin
    Cauquil, Laurent
    Bertide, Allan
    Ahn, Ingrid
    Barilly, Celine
    Gil, Lisa
    Canlet, Cecile
    Zemb, Olivier
    Pascal, Geraldine
    Samson, Arnaud
    Combes, Sylvie
    JOURNAL OF PROTEOME RESEARCH, 2021, 20 (01) : 982 - 994
  • [6] Effects of Early Intervention with Maternal Fecal Microbiota and Antibiotics on the Gut Microbiota and Metabolite Profiles of Piglets
    Lin, Chunhui
    Wan, Jiajia
    Su, Yong
    Zhu, Weiyun
    METABOLITES, 2018, 8 (04)
  • [7] The role of liver metabolism in compensatory-growth piglets induced by protein restriction and subsequent protein realimentation
    Zhang, M. Y.
    Hu, P.
    Feng, D.
    Zhu, Y. Z.
    Shi, Q.
    Wang, J.
    Zhu, W. Y.
    DOMESTIC ANIMAL ENDOCRINOLOGY, 2021, 74
  • [8] Effects of α-glycerol monolaurate on intestinal morphology, nutrient digestibility, serum profiles, and gut microbiota in weaned piglets
    Li, Longxian
    Wang, Huakai
    Zhang, Nan
    Zhang, Tuan
    Ma, Yongxi
    JOURNAL OF ANIMAL SCIENCE, 2022, 100 (03)
  • [9] Alterations in the diversity and composition of gut microbiota in weaned piglets infected with Balantioides coli
    He, Kai
    Yan, Wenchao
    Sun, Chenyang
    Liu, Jie
    Bai, Rongzhen
    Wang, Tianqi
    Qian, Weifeng
    VETERINARY PARASITOLOGY, 2020, 288
  • [10] Effects of Different Feed Additives on Intestinal Metabolite Composition of Weaned Piglets
    Zhao, Mingxuan
    Zhang, Jian
    Liu, Fuzhou
    Luo, Lv
    Wei, Mingbang
    Ye, Yourong
    Yangzom, Chamba
    Shang, Peng
    METABOLITES, 2024, 14 (03)