A Novel Type of Blood Biomarker: Distinct Changes of Cytokine-Induced STAT Phosphorylation in Blood T Cells Between Colorectal Cancer Patients and Healthy Individuals

被引:15
作者
Yun, Jae Won [1 ,2 ,3 ]
Lee, Sejoon [3 ,4 ]
Kim, Hye Mi [5 ]
Chun, Sejong [6 ,7 ]
Engleman, Edgar G. [8 ]
Kim, Hee Cheol [9 ]
Kang, Eun-Suk [1 ,10 ]
机构
[1] Sungkyunkwan Univ, Samsung Med Ctr, Sch Med, Dept Lab Med & Genet, Seoul 06351, South Korea
[2] Sungkyunkwan Univ, Samsung Adv Inst Hlth Sci & Technol, Seoul 06351, South Korea
[3] Samsung Med Ctr, Samsung Genome Inst, Seoul 06351, South Korea
[4] Seoul Natl Univ, Bundang Hosp, Dept Pathol, Seongnam Si 13620, Gyeonggi Do, South Korea
[5] Samsung Med Ctr, Samsung Biomed Res Inst, Seoul 06351, South Korea
[6] Chonnam Natl Univ, Dept Lab Med, Med Sch, Gwangju 61469, South Korea
[7] Chonnam Natl Univ, Hwasun Hosp, Gwangju 61469, South Korea
[8] Stanford Univ, Sch Med, Dept Pathol, Palo Alto, CA 94304 USA
[9] Sungkyunkwan Univ, Samsung Med Ctr, Sch Med, Dept Surg, Seoul 06351, South Korea
[10] Samsung Med Ctr, Stem Cell & Regenerat Med Inst, Seoul 06351, South Korea
关键词
STAT transcription factors; cytokines; tumor infiltrating lymphocytes; colorectal cancer; liquid biopsy; TUMOR; DISPARITIES; STRATEGIES;
D O I
10.3390/cancers11081157
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths worldwide. Although early diagnosis and treatment is the most successful strategy for improving patient survival, feasible and sensitive blood biomarkers for CRC screening remain elusive. Methods: Sixty-five CRC patients and thirty-three healthy individuals were enrolled. Peripheral blood (PB) and tumor tissues from CRC patients, and PB from healthy individuals were subjected to immunophenotyping and phospho-flow analysis of cytokine-induced phosphorylated STAT (CIPS). Logistic regression was used as a classifier that separates CRC patients from healthy individuals. Results: The proportion of regulatory T cells was increased in PB from CRC patients compared to PB from healthy individuals (p < 0.05). Interestingly, peripheral T cells share several cytokine-induced phosphorylated STAT (CIPS) signatures with T cells from CRC tumor-sites. Additionally, a classifier was made using two signatures distinct between T cells from CRC patients and T cells from healthy individuals. The AUCs (area under curves) of the classifier were 0.88 in initial cohort and 0.94 in the additional validation cohort. Overall AUC was 0.94 with sensitivity of 91% and specificity of 88%. Conclusion: This study highlights that immune cell signatures in peripheral blood could offer a new type of biomarker for CRC screening.
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页数:13
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