Enhancing grain-yield-related traits by CRISPR-Cas9 promoter editing of maize CLE genes

被引：263

作者：

Liu, Lei ^{[1
]}

Gallagher, Joseph ^{[2
]}

Arevalo, Edgar Demesa ^{[1
]}

Chen, Richelle ^{[1
]}

Skopelitis, Tara ^{[1
]}

Wu, Qingyu ^{[1
,3
]}

Bartlett, Madelaine ^{[2
]}

Jackson, David ^{[1
]}

机构：

[1] Cold Spring Harbor Lab, POB 100, Cold Spring Harbor, NY 11724 USA

[2] Univ Massachusetts, Biol Dept, Amherst, MA 01003 USA

[3] Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Beijing, Peoples R China

来源：

NATURE PLANTS | 2021年 / 7卷 / 03期

基金：

美国国家科学基金会;

关键词：

RECEPTOR-LIKE PROTEIN; POSTDOMESTICATION SPREAD; SHOOT; ARABIDOPSIS; ENCODES; DOMESTICATION; POPULATION; SELECTION; SOFTWARE; GENETICS;

D O I：

10.1038/s41477-021-00858-5

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Several yield-related traits selected during crop domestication and improvement(1,2) are associated with increases in meristem size(3), which is controlled by CLE peptide signals in the CLAVATA-WUSCHEL pathway(4-13). Here, we engineered quantitative variation for yield-related traits in maize by making weak promoter alleles of CLE genes, and a null allele of a newly identified partially redundant compensating CLE gene, using CRISPR-Cas9 genome editing. These strategies increased multiple maize grain-yield-related traits, supporting the enormous potential for genomic editing in crop enhancement.

引用

页码：287 / +

页数：10

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